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Clinical study of a new fecal occult blood test using a combination assay of hemoglobin and transferrin

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Summary

The applicability of a new immunological fecal occult blood test in which hemoglobin (Hb) and transferrin (Tf) are simultaneously assayed was evaluated. The mean absorbance and standard deviation (510/630 nm) obtained by this test was 0.840±0.805 in 51 fecal samples from patients with colon cancer, 0.248±0.305 in 95 samples from patients with colon polyps, and 0.104±0.053 in 110 samples from control patients; these values differed significantly (P<0.005). Hb and Tf concentrations were separately determined in the same fecal sampels, and qualitative evaluation was performed with a cutoff value of 5.1µg/g feces for Hb and 0.4µg/g feces for Tf. Hb or Tf was positive in 41 of the 51 samples in the colon cancer group, 33 of the 95 in the colon polyp group, and 3 of the 110 in the control group. Qualitative analysis of the values obtained by the combination assay of Hb and Tf with a cutoff value of 0.200 revealed positive rates of 41/51 in the colon cancer gorup, 33/95 in the colon polyp group, and 4/110 in the control group. These results sugest the usefulness of a combination assay of Hb and Tf as a fecal occult blood test.

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Authors and Affiliations

  1. The Second Department of Internal Medicine, Osaka Medical College, 2-7 Daigakucho, Takatsuki, 569, Osaka, Japan

    Hirofumi Miyoshi M.D., Takashi Amatsu, Chikao Shimamoto, Ichirou Hirata & Saburo Ohshiba

  2. Kyoto Medical Science Laboratory, Kyoto, Japan

    Kazuo Uchida & Ryoichi Matsuse

Authors
  1. Hirofumi Miyoshi M.D.
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  2. Kazuo Uchida
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  3. Ryoichi Matsuse
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  4. Takashi Amatsu
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  5. Chikao Shimamoto
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  6. Ichirou Hirata
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  7. Saburo Ohshiba
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Miyoshi, H., Uchida, K., Matsuse, R. et al. Clinical study of a new fecal occult blood test using a combination assay of hemoglobin and transferrin. Gastroenterol Jpn 26, 151–156 (1991). https://doi.org/10.1007/BF02811073

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  • DOI: https://doi.org/10.1007/BF02811073

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