Abstract
Genetic diversity in a sample of an aquatic plantButomus umbellatus from 37 localities in Czechia and Slovakia was studied by analyzing six polymorphic loci in three enzymatic systems (SKDH, PGD and AAT). Diversity among ramets was low in eight populations with relatively extensive sampling (only one population possessed more than one multilocus genotype), suggesting high clonality of reproduction in these populations. However, among-population diversity was high: G = 0.782 and 0.881 for the samples of diploid and triploid populations, respectively. Heterozygosity of individual plants averaged over variable loci was also high: H = 0.554 for diploids and 0.453 for triploids. Genetic differentiation among populations was additionally studied using cluster analysis. Several populations of diploids clustered separately from all other populations, whereas another group of diploid populations clustered with some triploid populations, indicating the possibility of relatively recent, probably multiple origin of these triploid populations from their diploid progenitors. Association between matrices of Nei’s genetic distances among populations from different localities and matrices of geographic distances among these localities revealed highly significant correlation for the sample of diploid populations (r = 0.60,P < 0.001) but no significant correlation for the sample of triploid populations (r = 0.02,P = 0.593). These results indicate a spatial structure of diploid populations in accordance with the isolation by distance model, and a random distribution of genotypes among triploid populations ofB. umbellatus.
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References
Aspinwall N. &Christian T. (1992): Clonal structure, genotypic diversity, and seed production in populations ofFilipendula rubra (Rosaceae) from the Northcentral United States.Amer. J. Bot. 79: 294–299.
Barrett S.C.H., Eckert C.G. &Husband B.C. (1993): Evolutionary processes in aquatic plant populations.Aquatic Bot. 44: 105–145.
Bartish I.V., Jeppsson N., Bartish G.I., Lu R. &Nybom H. (2000): Inter- and intraspecific genetic variation inHippophae (Eleagnaceae) investigated by RAPD markers.Pl. Syst. Evol. 225: 85–101.
Eckert C.G. &Barrett S.C.H. (1993): Clonal reproduction and patterns of genotypic diversity inDecodon verticillatus (Lythraceae).Amer. J. Bot. 80: 1175–1182.
Eckert C.G., Lui K., Bronson K., Corradini P. &Bruneau A. (2003): Population genetic consequences of extreme variation in sexual and clonal reproduction in an aquatic plant.Molec. Ecol. 12: 331–334.
Ellstrand N.C. &Rose M.L. (1987): Patterns in genotypic diversity in clonal plant species.Amer. J. Bot. 74: 123–131.
Fernando D.D. &Cass D.D. (1996): Genotypic differentiation inButomus umbellatus (Butomaceae) using isozymes and random amplified polymorphic DNAs.Canad. J. Bot. 74: 647–652.
Hroudová Z. &Zákravský P. (1993): Ecology of two cytotypes ofButomus umbellatus. III. Distribution and habitat differentiation in the Czech and Slovak Republics.Folia Geobot. Phytotax. 28: 425–435.
Kirschner J. (1995): Allozyme analysis ofLuzula sect.Luzula (Juncaceae) in Ireland: Evidence of the origin of tetraploids.Folia Geobot. Phytotax. 30: 283–290.
Kirschner J., Kirschnerová L., Štepánek J. &Tichý M. (1994):Analýza isoenzymů v populační biologii rostlin (Isozyme analysis in plant population biology). Institute of Botany AS CR, Průhonice.
Kirvan A.P. (1997):Latitude/Longitude, NCGIA Core Curriculum in GI Science. http://www.ncgia.ucsb.edu/giscc/ units/u014/u014.html, posted October 29,1997.
Krahulcová A. &Jarolímová V. (1993): Ecology of two cytotypes ofButomus umbellatus. I. Karyology and breeding behaviour.Folia Geobot. Phytotax. 28: 385–411.
Nybom H. &Bartish I.V. (2000): Effects of life history traits and sampling strategies on genetic diversity estimates obtained with RAPD markers in plants.Perspectives Pl. Ecol. Evol. Syst. 3: 93–114.
Piquot Y., Saumitou-Larpade P., Petit D., Vernet P. &Epplen J.T. (1996): Genotypic diversity revealed by allozymes and oligonucleotide DNA fingerprinting in French populations of the aquatic macrophyteSparganium erectum.Molec. Ecol. 5: 251–258.
Oden N.L. &Sokal R.R. (1986): Directional autocorrelation: An extension of spatial correlograms to two dimension.Syst. Zool. 35: 608–617.
Rohlf F.J. (1997):NTSYS/pc. Numerical taxonomy and multivariate analysis system. Version 1.80. Exeter Software, Setauket.
Slatkin M. (1993): Isolation by distance in equilibrium and non-equilibrium populations.Evolution 47: 264–279.
Vallejos C.E. (1983): Enzyme activity staining. In:Tanksley S.D. &Orton T.J. (eds.),Isozymes in plant genetics and breeding, Part A, Elsevier, Amsterdam, pp. 469–516.
Widén B., Cronberg N. &Widén M. (1994): Genotypic diversity, molecular markers and spatial distribution of genetic variation in clonal plants, a literature survey.Folia Geobot. Phytotax. 29: 245–263.
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Kirschner, J., Bartish, I., Hroudová, Z. et al. Contrasting patterns of spatial genetic structure of diploid and triploid populations of the clonal aquatic species,Butomus umbellatus (Butomaceae), in Central Europe. Folia Geobot 39, 13–26 (2004). https://doi.org/10.1007/BF02803261
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DOI: https://doi.org/10.1007/BF02803261