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Radioimmunoassay of progesterone and estriol in plasma using antibodies immobilized onto protein A-sepharose CL-4B

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Abstract

Protein A-Sepharose CL-4B was used as a solid phase for antibodies in the radioimmunoassay of progesterone and estriol. The method was fast and easily standardizable. Immobilized antibodies had the same binding capacity as free antibodies and gave good correlation curves (r = 0.996 for progesterone andr = 0.989 for estriol). Sensitivity was 12.5 pg/tube for progesterone and 8.0 pg/tube for estriol. Comparison of progesterone radioimmunoassay with chemically immobilized antibody onto Sepharose CL-4B was also carried out.

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Authors and Affiliations

  1. Istituto di Chimica degli Ormoni, CNR, Via Mario Bianco 9, 20131, Milano, Italy

    Maria Angela Bacigalupo & Giacomo Carrea

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  1. Maria Angela Bacigalupo
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  2. Giacomo Carrea
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Bacigalupo, M.A., Carrea, G. Radioimmunoassay of progesterone and estriol in plasma using antibodies immobilized onto protein A-sepharose CL-4B. Appl Biochem Biotechnol 11, 279–285 (1985). https://doi.org/10.1007/BF02798442

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  • DOI: https://doi.org/10.1007/BF02798442

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