Summary
Population doublings versus time in culture were compared in human postnatal skin fibroblasts from normal donors, a cancer patient, and from donors suffering from Cockayne syndrome, Ataxia telangiectasia, and Fanconi’s anemia (FA). Confluent cultures were maintained in a nonproliferating state for 14 to 27 d in 0.5% serum medium. The results show that the ability of cells to resume division after a resting stage can be influenced by pathologic conditions. In arrested FA cell populations an increase of the population doublings and of the calendar time were observed. It is possible that in some cell populations the resting stage favors the expression of growth potentialities related to an instability of the cells.
Similar content being viewed by others
References
Hayflick, L.; Moorhead, P. S. The serial cultivation of human diploid cell strains. Exp. Cell Res. 25: 585–621; 1961.
Hay, R. J.; Menzies, R. A.; Morgan, H. P.; Strehler, B. L. The division potential of cells in continuous growth as compared to cells subcultivated after maintenance in stationary phase. Exp. Gerontol. 3: 35–44; 1968.
Hay, R. J.; Strehler, B. L. The limited growth span of cell strains isolated from chick embryo. Exp. Gerontol. (2) 123: 135; 1967.
McHale, J. S.; Mouton, M. L.; McHale, J. T. Limited culture lifespan of human diploid cells as a function of metabolic time instead of division potential. Exp. Gerontol. 6: 89–93; 1971.
Dell’Orco, R. T.; Mertens, J. G.; Kruse, P. F., Jr. Doubling potential, calendar time and senescence of human diploid cells in culture. Exp. Cell Res. 77: 356–360; 1973.
Goldstein, S.; Singal, D. P. Senescence of cultured human fibroblasts: mitotic versus metabolic time. Exp. Cell Res. 88: 359–364; 1974.
Ryan, J. M. Cellular senescence: calendar time versus division potential. Gerontologist 17: 113; 1977.
Nielsen, P. J.; Ryan, J. M. Cumulative population doublings as the determinant of chick cell life span. In Vitro 6: 237; 1980.
Roberts, T. W.; Smith, J. R. The proliferative potential of chick embryo fibroblasts: population doublings versus time in culture. Cell Biol. Int. Rep. 4: 1057–1063; 1980.
Cristofalo, V. J.; Palazzo, R.; Charpentier, R. Chronologic versus replicative life span in human cell cultures: a reexamination. In Vitro 6: 59; 1980.
Dell’Orco, R. T.; Mertens, J. G.; Kruse, P. F., Jr. Doubling potential, calendar time and donor age of human diploid cells in culture. Exp. Cell Res. 84: 363–366; 1974.
Kaji, K.; Matsuo, M. Doubling potential and calendar time of human diploid cells in vitro. Exp. Gerontol. 14: 329–334; 1979.
Kaji, K.; Matsuo, M. A low density inoculation method for the serial subcultivation of human diploid fibroblasts: an efficient model system for the study of cellular ageing. Mech. Age. Dev. 13: 219–225; 1980.
Macieira-Coelho, A. Influence of cell density on growth inhibition of human fibroblasts in vitro. Proc. Soc. Exp. Biol. Med. 125: 548–552; 1967.
Fogh, J.; Fogh, H. A method for direct demonstration of pleuropneumonia-like organisms in cultured cells. Proc. Soc. Exp. Biol. Med. 117: 899–901; 1964.
Guzzetta, F. Cockayne-Neil-Dingwall syndrome. Vinken, P. J.; Bruyen, G. W. eds. Handbook of clinical neurology, vol. 3. Amsterdam: North Holland Publishing Corp.; 1972: 431–440.
Schmickel, R. D.; Chu, E. H. Y.; Trosko, J. E.; Chang, C. C. Cockayne syndrome: a cellular sensitivity to ultraviolet light. Pediatrics 60: 135–139; 1977.
Deschavanne, P. J.; Diatloff-Zito, C.; Macieira-Coelho, A.; Malaise, E. P. Unusual sensitivity of two Cockayne syndrome cell lines to both UV and gamma-irradiation. Mutat. Res. 91: 403–406; 1981.
Paterson, M. C.; Smith, P. J. Ataxia telangiectasia, an inherited human disorder involving hypersensitivity to ionizing radiation and related DNA-damaging chemicals. Ann. Rev. Genet. 13: 291–318; 1979.
Todaro, G. J.; Green, H.; Swift, M. R. Susceptibility of human diploid fibroblast strains to transformation by SV-40 virus. Science 153: 1252–1254; 1966.
Auerbach, A. D.; Wolman, S. R.; Chaganti, R. S. K. A spontaneous clone of Fanconi’s anemia fibroblasts with chromosome abnormalities and increased growth potential. Cytogenet. Cell Genet. 28: 265–270; 1980.
Macieira-Coelho, A.; Diatloff, C.; Billard, M.; Fertil, B.; Malaise, E.; Fries, D. Effect of low dose rate irradiation on the division potential of cells in vitro. IV — Embryonic and adult human lung fibroblast-like cells. J. Cell. Physiol. 95: 235–238; 1978.
Diatloff, C.; Macieira-Coelho, A. Effect of low dose rate irradiation on the division potential of cells in vitro. V — Human skin fibroblasts from donors with a high risk of cancer. J. Natl. Cancer Inst. 63: 55–59; 1979.
Azzarone, B.; Diatloff-Zito, C.; Billard, C.; Macieira-Coelho, A. Effect of low dose rate irradiation on the division potential of cells in vitro. VII — Human fibroblasts from young and adult donors. In Vitro 16: 634–638; 1980.
Author information
Authors and Affiliations
Additional information
This work was supported by contract CRL 802030 from the I.N.S.E.R.M. and by Euratom.
Rights and permissions
About this article
Cite this article
Diatloff-Zito, C., Macieira-Coelho, A. Effect of growth arrest on the doubling potential of human fibroblasts in vitro: A possible influence of the donor. In Vitro Cell.Dev.Biol. -Plant 18, 606–610 (1982). https://doi.org/10.1007/BF02796392
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02796392