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A technique for developing established cell lines from human osteosarcomas

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Summary

A method is described which has been successfully used to develop two human osteogenic sarcomas into established lines in culture. This method provides a means whereby cells growing from explanted tumor tissue can be immediately cloned and the fibroblastic (nonneoplastic) cells thus selected against. Both lines have been passaged for over 100 population doublings since cloning and have retained the ability to form colonies from single cells plated at low density without the use of feeder layers or conditioned medium. In culture, the osteogenic sarcoma cells are nonfibroblastic, pile up, and appear to retain a morphological similarity to the in vivo tumors from which they were derived. A karyotype of cells derived from one of the tumors containing a marker chromosome is also presented.

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References

  1. Giard, D. J., S. A. Aaronson, G. J. Todaro, et al. 1973.In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417–1421.

    PubMed  CAS  Google Scholar 

  2. Evans, V. J., and W. F. Anderson. 1966. Effect of serum on spontaneous neoplastic transformationsin vitro. J. Natl. Cancer Inst. 37: 247–249.

    PubMed  CAS  Google Scholar 

  3. Parshad, R., and K. K. Sanford. 1968. Effect of horse serum, fetal calf serum, bovine serum, and fetuin on neoplastic conversion and chromosomes of mouse embryo cellsin vitro. J. Natl. Cancer Inst. 41: 767–779.

    PubMed  CAS  Google Scholar 

  4. Stoker, M. 1968. Abortive transformation by polyoma virus. Nature. 218: 234–238.

    Article  PubMed  CAS  Google Scholar 

  5. Saksela, E., and P. S. Moorehead. 1963. Aneuploidy in the degenerative phase of serial cultivation of human cell strains. Proc. Natl. Acad. Sci. U.S.A. 50: 390–395.

    Article  PubMed  CAS  Google Scholar 

  6. Maunoury, R., J. Arnoult, and C. Vedrenne. 1972. Etablissement et characterisation d’une lignee cellulaire provenant d’un osteosarcome humain. Pathol. Biol. (Paris). 20: 369–376.

    CAS  Google Scholar 

  7. McAllister, R. M., M. B. Gardner, A. E. Greene, et al. 1970. Cultivationin vitro of cells derived from a human osteosarcoma. Cancer 27: 397–402.

    Article  Google Scholar 

  8. Giraldo, G., E. Beth, Y. Hirshaut, et al. 1971. Human sarcomas in culture. Foci of altered cells and a common antigen; induction of foci and antigen in human fibroblast cultures by filtrates. J. Exp. Med. 133: 454–478.

    Article  PubMed  CAS  Google Scholar 

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Author notes

  1. J. Epstein

    Present address: Department of Microbiology and Molecular Genetics, Harvard Medical School, and the Clinical Genetics Unit, Department of Medicine, Children’s Hospital Medical Center, 02115, Boston, Mass.

Authors and Affiliations

  1. Laboratory of Radiobiology, Department of Physiology, Harvard University School of Public Health, 665 Huntington Avenue, 02115, Boston, Massachusetts

    Ralph Weichselbaum, J. Epstein & John B. Little

  2. Joint Center for Radiation Therapy, and the Department of Radiation Therapy, Harvard Medical School, 02115, Boston, Mass

    Ralph Weichselbaum

Authors
  1. Ralph Weichselbaum
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  2. J. Epstein
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  3. John B. Little
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Additional information

This work was supported by grants CA-05237 and CA-11751 from the National Cancer Institute, and grant ES-00002 from the National Institute of Environmental Health Sciences.

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Weichselbaum, R., Epstein, J. & Little, J.B. A technique for developing established cell lines from human osteosarcomas. In Vitro Cell.Dev.Biol.-Plant 12, 833–836 (1976). https://doi.org/10.1007/BF02796368

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  • DOI: https://doi.org/10.1007/BF02796368

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