In Vitro - Plant

, Volume 18, Issue 10, pp 827–834 | Cite as

Establishment and characterization of chicken embryo fibroblast clone LSCC-H32

  • O. -R. Kaaden
  • Susanne Lange
  • B. Stiburek


Cell line CEC-32 and clone LSCC-H32 were established from primary chicken embryo cells spontaneously but not experimentally transformed at 32° C. The lines consisted of fibroblastoid and polygonal cells and had a subtetraploid karyotype of 2N=130 to 140. The cells showed increased plating efficiency and metabolic activities as demonstrated by hexose uptake and plasminogen activator assay. The established cells produced avian lymphoid leukosis viruses of subgroups A and B. The virus released from LSCC-H32 cells induced lymphoid leukosis in inoculated chickens 18 to 22 wk post infection (PI). The cells have been carried in continuous culture for 285 passages and they appeared to grow indefinitely. They were efficiently used to propagate several animal viruses and to titrate chicken interferon.

Key words

chicken embryo cell line avian retrovirus 


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  1. 1.
    Hihara, H.; Shimizu, T.; Yamamoto, H. Cultivation and some properties of tumor cells from chickens with avian lymphoid leukosis. Jpn. J. Vet. Sci., Suppl. 168; 1970.Google Scholar
  2. 2.
    Siegfried, L. M.; Olson, C. Characteristics of the avian transmissible lymphoid tumor cells maintained in culture. J. Natl. Cancer Inst. 48: 791–795; 1972.PubMedGoogle Scholar
  3. 3.
    Akiyama, Y.; Kato, S. Two cell lines from lymphoma of Marek’s. Biken J. 17: 105–116; 1974.PubMedGoogle Scholar
  4. 4.
    Powell, P. C.; Payne, L. N.; Frazier, J. A.; Rennie, A. Tlymphoblastoid cell line from Marek’s disease lymphomas. Nature 251: 79–80; 1974.PubMedCrossRefGoogle Scholar
  5. 5.
    Beug, H.; Graf, Th. Isolation of clonal strains of chicken embryo fibroblasts. Exp. Cell Res. 107: 417–428; 1977.PubMedCrossRefGoogle Scholar
  6. 6.
    Gey, G. O.; Svotelis, M.; Foard, M.; Bang, F. B. Long-term growth of chicken embryo fibroblasts on a collagen substrate. Exp. Cell Res. 84: 63–71; 1974.PubMedCrossRefGoogle Scholar
  7. 7.
    Witter, R. L. et al. A proposed method for designating avian cell lines and transplantable tumors. Avian Pathol. 8: 487–498; 1979.CrossRefGoogle Scholar
  8. 8.
    Langlois, A. M.; Ishizaki, R.; Beaudreau, G. S.; Kummer, J. F.; Beard, J. W.; Bolognesi, D. P. Virus-infected avian cell lines established in vitro. Cancer Res. 36: 3884–3904; 1976.Google Scholar
  9. 9.
    Dinowitz, M. A continuous line of Rous sarcoma virus-transformed chick embryo cells. J. Natl. Cancer Inst. 58: 307–312; 1977.PubMedGoogle Scholar
  10. 10.
    Franklin, R. B.; Kang, C. Y.; van Katherine, M. M.; Bose, H. R., Jr. Transformation of chick embryo fibroblasts by reticuloendotheliosis virus. Virology 8: 313–321; 1977.CrossRefGoogle Scholar
  11. 11.
    Rubin, H. A substance in conditioned medium which enhances the growth of small numbers of chick embryo cells. Exp. Cell Res. 41: 138–148; 1966.PubMedCrossRefGoogle Scholar
  12. 12.
    Moorhead, P. S.; Nowell, P. C.; Mellmann, W. J.; Hungerford, D. A. Chromosome preparations in leukocytes cultured from human peripheral blood. Exp. Cell Res. 20: 613–615; 1960.PubMedCrossRefGoogle Scholar
  13. 13.
    Rifkin, D. B.; Pollack, R. E. Production of plasminogen activator by established cell lines of mouse origin. J. Cell. Biol. 73: 47–55; 1977.PubMedCrossRefGoogle Scholar
  14. 14.
    Martin, S. S.; Venuta, S.; Weber, M.; Rubin, H. Temperature-dependent alterations in sugar transport in cells infected by a temperature-sensitive mutant of RSV. Proc. Natl. Acad. Sci. USA 68: 2739–2741; 1971.PubMedCrossRefGoogle Scholar
  15. 15.
    Boscheck, C. B.; Jockusch, B. M.; Friis, R. R.; Back, R.; Grundmann, E.; Bauer, H. Early changes in the distribution and organization of microfilament proteins during cell transformation. Cell 24: 175–184; 1981.CrossRefGoogle Scholar
  16. 16.
    Chubb, R. E.; Churchill, A. E. Precipitating antibodies associated with Marek’s disease. Vet. Rec. 83: 4–7; 1968.Google Scholar
  17. 17.
    Crittenden, L. B.; Eagen, D. A.; Gulvas, F. A. Assays for endogenous and exogenous lymphoid leukosis viruses and chick helper factor with RSV (−) cell lines. Infect. Immun. 24: 379–386; 1979.PubMedGoogle Scholar
  18. 18.
    Okazaki, W.; Purchase, H. G.; Burmester, B. R. Phenotypic mixing test to detect and assay avian leukosis viruses. Avian Dis. 19: 311–317; 1975.PubMedCrossRefGoogle Scholar
  19. 19.
    Bauer, G.; Temin, H. M. Pheasant virus DNA polymerase is related to avian leukosis virus DNA polymerase at the active site. J. Virol. 32: 78–90; 1979.PubMedGoogle Scholar
  20. 20.
    Vogt, P. K. Focus assay of Rous sarcoma virus. Habel, K.; Salzman, N., eds. Fundamental techniques in virology. New York: Academic Press; 1969; 198–211.Google Scholar
  21. 21.
    Lowry, O. M.; Rosebrough, N. J.; Farr, A. L.; Randall, R. J. Protein measurements with the folin phenol reagent. J. Biol. Chem. 193: 265–275; 1951.PubMedGoogle Scholar
  22. 22.
    Edelmann, G. M.; Yahara, I. Temperature-sensitive changes in the surface modulation assemblies of fibroblasts transformed by mutants of Rous sarcoma virus. Proc. Natl. Acad. Sci. USA 73: 2047–2051; 1976.CrossRefGoogle Scholar
  23. 23.
    Graf, Th.; Beug, H. Avian leukemia viruses. Interaction with their target cells in vivo and in vitro. Biochem. Biophys. Acta 516: 269–299; 1978.PubMedGoogle Scholar
  24. 24.
    Oker-Blom, N.; Kallio, A.; Hartling, L. Morphological changes in chick embryo cell cultures induced by avian leukosis viruses. Intervirology 5: 342–353; 1975.PubMedGoogle Scholar
  25. 25.
    Payne, L. N.; Biggs, P. M. Genetic resistance of fowl to MH2 reticuloendotheliosis virus. J. Gen. Virol. 7: 177–185; 1970.PubMedCrossRefGoogle Scholar

Copyright information

© Tissue Culture Assoc. Inc. 1982

Authors and Affiliations

  • O. -R. Kaaden
    • 1
  • Susanne Lange
    • 1
  • B. Stiburek
    • 1
  1. 1.Hannover School of Veterinary MedicineInstitute for Virology and Clinic for PoultryHannoverGermany

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