Abstract
The effectiveness of chemiluminescence (ChL) in vitro to measure free radicals generated as a result of metabolic disorganization caused by radiation sickness is evaluated. The results are correlated with those obtained by measuring superoxide dismutase (SOD) activity and lipid peroxide as levels of thiobarbituric acid reacting substances (TBARS).
To this aim, livers from irradiated Wistar rats were removed immediately (day 0) after irradiation and also 7 and 14 d later. ChL results, expressed in arbitrary units (AU)/min/mg protein, were analyzed for irradiated samples and controls, for different doses at different times. Increased levels of ChL emission were observed not only on day 0, but also on days 7 and 14. On the other hand, SOD activity showed a decrease on the 7th d, and significantly higher lipid peroxide levels were observed in the assays performed on the 14th d, at all exposure doses. The correlation between temporal changes in the SOD activity, ChL emission, and higher TBARS levels a week later were evident from the data.
These results indicate that the ChL technique proved to be useful in combination with other techniques currently used for evaluating radiation oxidative injury.
Similar content being viewed by others
References
R. Gerschmman,Proc. Intern. Congr. Physiol. Sci. 21st, Buenos Aires, 222–226 (1959).
A. Boveris, C. Fraga, A. Varsavsky,Arch Biochem Biophys. 199, 438–447 (1983).
M. Nakano, T. Noguchi, K. Sugioka, et al.J Biol Chem. 250, 2404–2406 (1975).
A. Petkau,Br J Cancer. 55, Suppl. VIII 87–95 (1987).
A. Konings, S. Oosterloo,Rad Res. 81, 200–207 (1980).
A. Boveris, E. Cadenas, R. Reiter, et al.Proc Natl Acad Sci USA 77, 347–351 (1980).
O. Lowry, N. Rosebrough, A. Farr, et al.J Biol Chem. 193, 265–275 (1951).
H. Misra, I. Fridovich,J Biol Chem. 247, 3170–3175 (1972).
H. Ohkawa, O. Nobuko, Y. Kunio,Anal Biochem. 95, 351–358 (1979).
E. Cadenas, A. Boveris, B. Chance,Biochem J. 186, 659–667 (1980).
A. Adamo, S. Llesuy, J. Pasquini, A. Boveris,Biochem J. 263, 273–277 (1989)
A. Petkau,Photochem Photobiol. 28, 765 (1978).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Dubner, D., Gisone, P., Jaitovich, I. et al. Free radicals production and estimation of oxidative stress related to γ irradiation. Biol Trace Elem Res 47, 265–270 (1995). https://doi.org/10.1007/BF02790126
Issue Date:
DOI: https://doi.org/10.1007/BF02790126