Abstract
Several bacterial cell wall proteins, like proteins A and G, with valuable affinity for immunoglobulins have been discovered and are currently employed in immunological techniques. In 1988, protein L, a bacterial cell wall protein with Ig-binding capacity, was isolated from the anaerobic bacterial speciesPeptostreptococcus magnus. Binding data with immunoglobulin fragments suggested that protein L could selectively bind the variable region of human kappa light chains. More recently a recombinant LG fusion protein was expressed inE. coli containing four repeated Ig-binding domains of protein L (fragment B1–4) and two IgG Fc-binding protein G domains (fragment CDC). Recombinant L and LG proteins were tested in the purification of murine monoclonal IgG and their fragments. After affinity-constant evaluation in different buffer systems, high-pressure affinity-chromatography columns were prepared by coupling the proteins to Affi-prep 10 resin and tested with eight different murine monoclonal antibodies and their fragments of various isotypes. Affinity-chromatography experiments confirming radioimmunoassay results showed 75% fragment-binding capacity of protein L and 100% of protein LG. These results evidenced protein LG as the most powerful Ig-binding tool so far described. Therefore, application of these proteins may be suggested in the purification of murine immunoglobulins and their fragments, including the engineered ones.
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Forgsen, A. and Sjoquist, J. (1966)J. Immunol. 97, 822–827.
Björck, L. and Kronvall, G. (1984)J. Immunol. 133, 969–974.
Fahnestock, S. R., Alexander, P., Nagh, J., and Filipula, D. (1986)J. Bacteriol. 167, 870–880.
Guss, B., Eliassen, M., Olsson, A., Uhlen, M., Frej, A. K., Jornvall, M., et al. (1986)EMBO J. 5, 1567–1575.
Björck, L. (1988)J. Immunol. 140, 1194–1197.
Kastern, W., Sjöbring, U., and Björck, L. (1992)J. Biol. Chem. 267, 12820–12825.
Kihlberg, B. M., Sjöbring, U., Kastern, V., and Björck, L. (1992)J. Biol. Chem. 267.
Colcher, D., Horan-Hand, P., Nuti, M., and Schlom, J. (1981)Proc. Natl. Acad. Sci. USA 78, 3199–3203.
Buraggi, G. L., Callegaro, L., Turrin, A., Gennari, L., Bombardieri, E., Mariani, G., et al. (1984)Cancer Detect. Prevent. 10, 335–345.
Prat, M., Morra, I., Bussolati, G., and Comoglio, P. M. (1985)Cancer Res. 45, 5799–5807.
Miotti, S., Canevari, S., Menard, S., Mezzanzanica, D., Porro, D., Pupa, S. M., et al. (1987)Int. J. Cancer 39, 297–303.
Wilson, B. S., Imai, K., Natali, P. G., and Ferrone, S. (1981)Int. J. Cancer 28, 292–300.
Muraro, R., Nuti, M., Natali, P. G., Biagiotti, A., Simpson, J. F., Primus, F. J., et al. (1989)Int. J. Cancer 43, 598–607.
Mariani, M., Camagna, M., Tarditi, L., and Seccamani, E. (1991)Mol. Immunol. 28, 69–77.
Zaccolo, M. and Malavasi, F. (1993)Int. J. Clin. Lab. Res. 23.
Harper, M., Lema, F., Boulot, G., and Poljack, R. J. (1987)Mol. Immunol. 24, 97–108.
Smith-Gill, S. J., Mainhart, C. R., Lavoie, T. B., Rudikoff, S., and Potter, M. (1984)J. Immunol. 132, 963.
Fraker, P. J. and Speck, J. C. Jr. (1978)Biochem. Biophys. Res. Commun. 80, 849–857.
Antoni G. and Mariani, M. (1985)J. Immunol. Meth. 83, 61–68.
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Vola, R., Lombardi, A., Tarditi, L. et al. Recombinant proteins L and LG. Cell Biophysics 24, 27–36 (1994). https://doi.org/10.1007/BF02789212
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DOI: https://doi.org/10.1007/BF02789212