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Gene targeting of retinoid receptors

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Abstract

Gene targeting in embryonic stem (ES) cells has been employed to investigate the role of the retinoid receptors and binding proteins both in the mouse as well as in embryocarcinoma cells. It is a powerful technique for the modification of the mouse genome. With more recent refinements in gene targeting technology, it is now possible to introduce more subtle mutations in the murine genome, as well as to investigate gene function in a tissue and temporally-restricted manner. It should also be possible to modify genes in diverse diploid cell lines, to generate diverse model systems for analysis of retinoid receptor function. In this article, some of the basic principles for gene targeting are described.

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  1. Institut de Rechercheo Cliniques, 110 ave des Pins ouest, H2W IR7, Montreal, Quebec, Canada

    David Lohnes

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  1. David Lohnes
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Lohnes, D. Gene targeting of retinoid receptors. Mol Biotechnol 11, 67–84 (1999). https://doi.org/10.1007/BF02789177

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