Abstract
Rat germline-competent embryonic stem (ES) cell lines have been available since 2008, and rat models with targeted mutations have been successfully generated using ES cell-based genome targeting technology. This chapter will focus on the procedures of gene targeting in rat ES cells.
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Acknowledgements
This work was supported by a grant from National Institutes of Health: P40 OD011062 (ECB).
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Men, H., Davis, D.J., Bryda, E.C. (2023). Gene Targeting in Rat Embryonic Stem Cells. In: Saunders, T.L. (eds) Transgenesis. Methods in Molecular Biology, vol 2631. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2990-1_15
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DOI: https://doi.org/10.1007/978-1-0716-2990-1_15
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-0716-2990-1
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