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TransgenicLycium barbarum L. Established as HIV capsid protein expression system

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Abstract

A DNA fragment (MA4-CA) encoding HIV CA viral like particles (VLPs) was transferred intoLycium barbarum L. byAgrobacterium tumefaciens-mediated transformation. PCR amplification analysis of DNA from transgenic plants confirmed the presence of the fusion gene under the control of promoter p35S. CA protein was identified in transformed leaf extracts by enzyme-linked immunosorbent assay (ELISA), indicating that a transgenicLycium barbarum L. expression system can produce immunogenic CA.

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Abbreviations

CA:

capsid

VLPs:

virus-like particles

MA4-CA:

matrix-capsid fusion protein

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Authors and Affiliations

  1. Key Laboratory for Biotech-Drugs Ministry of Health, Shandong Medical Biotechnology center, 250062, Ji'nan, China

    Guo-Li Du, Chang-Zheng Song, Geng-Lin Zhang, Xiao-Guang Sun & Dan-Ru Liu

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  1. Guo-Li Du
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  2. Chang-Zheng Song
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  3. Geng-Lin Zhang
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  4. Xiao-Guang Sun
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  5. Dan-Ru Liu
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Corresponding author

Correspondence to Chang-Zheng Song.

Additional information

Supported by the Natural Science Foundation of China, Nos. 30271215 and 30471621

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Du, GL., Song, CZ., Zhang, GL. et al. TransgenicLycium barbarum L. Established as HIV capsid protein expression system. Plant Mol Biol Rep 23, 411–416 (2005). https://doi.org/10.1007/BF02788889

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