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Identification of lectin activity in the hemolymph ofCastnia licus Drury, a sugar-cane giant borer (Lepidoptera-Castniidae)

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Abstract

Castnia licus Drury, in the larvae stage, is a major pest in the sugarcane industry of Northeastern Brazil (giant borer). A natural hemagglutination activity was detected in the larvae hemolymph of this insect and the activity have not been inhibited by tested carbohydrates. Fractionation with ammonium sulfate showed that the supernatant of the 15–30% fraction (S15-30%) had the lectin’s highest specific activity. Acidic and basic lectin activities were separated by chromatography on CM-cellulose; the fractionated molecular forms showed distinct electrophoresis mobilities.

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Authors and Affiliations

  1. Departamento de CiÊncias Básicas e Sociais, Centro de FormaÇÃo de Tecnólogos, Universidade Federal da Paraiba (UFPB), Campus IV, Bananeiras-PB

    Roberto V. Antunes

  2. Departamento de Bioquímica, Centro de CiÊncias Biológicas, Universidade Federal de Pernambuco (UFPE), Av.Moraes Rego, s/n, Cidade Universitária, 50730, Recife-PE, Brazil

    Luana C. B. B. Coelho

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  1. Roberto V. Antunes
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  2. Luana C. B. B. Coelho
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Antunes, R.V., Coelho, L.C.B.B. Identification of lectin activity in the hemolymph ofCastnia licus Drury, a sugar-cane giant borer (Lepidoptera-Castniidae). Appl Biochem Biotechnol 47, 33–37 (1994). https://doi.org/10.1007/BF02788673

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  • DOI: https://doi.org/10.1007/BF02788673

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