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In vitro and in vivo effects of selenium and selenium with vitamin E on platelet functions in diabetic rats relationship to platelet sorbitol and fatty acid distribution

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Abstract

In vitro 30 min of incubation with selenomethionine (Sm)+vitamin E multiplied by about five platelet selenium (Se) decreased significantly platelet thrombin and ADP-induced aggregation decrease. Four groups of streptozotocin-induced diabetic rats were fed with a supplemented purified diet with an Se-rich yeast (Selenion): DSel, Sm: DSm, Sm α-tocopherol: DSmE or unsupplemented diet: D. After 24 wk of supplementation, only a decrease in thrombin-induced aggregation in group DSel compared to DSm and DSmE and D was observed. However, after 24 wk of diet compared to 14 wk, in group D and DSm, a significant increase in thrombin-induced aggregation occurred (p<0.0001), whereas a significant decrease in groups DSel and DSmE (p<0.0001,p<0.03) was noted. After 21 wk of diet, in DSmE, platelet adhesion to fibronectin was significantly decreased compared to group D (p<0.05). These changes in DSmE were associated with a significant decrease in platelet sorbitol (p<0.02) and a very significant increase in platelet Se (p<0.0005). Sm associated with vitamin E would appear more efficient to prevent oxidative damage of diabetic platelet membrane and thus to modulate its hyperactivity.

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Authors and Affiliations

  1. Unit 331, National Institute of Health and Medical Research, Bron, France

    Christelle Douillet & Maryvonne Ciavatti

  2. Trace Elements Institute for UNESCO, Lyon, France

    Muriel Bost

  3. Department of Pharmacological Biochemistry, E. Herriot Hospital, Lyon, France

    Michèle Accominotti

  4. Department of Endocrinology, Antiquaille Hospital, Lyon, France

    Françoise Borson-Chazot

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  1. Christelle Douillet
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  2. Muriel Bost
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  3. Michèle Accominotti
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  4. Françoise Borson-Chazot
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  5. Maryvonne Ciavatti
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Douillet, C., Bost, M., Accominotti, M. et al. In vitro and in vivo effects of selenium and selenium with vitamin E on platelet functions in diabetic rats relationship to platelet sorbitol and fatty acid distribution. Biol Trace Elem Res 55, 263–277 (1996). https://doi.org/10.1007/BF02785285

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  • DOI: https://doi.org/10.1007/BF02785285

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