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Induction and characterization of metallothionein in different organs ofOstrea edulis L.

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Abstract

In this study, the induction of metallothionein (MT) in one species of oysterOstrea edulis exposed to copper (Cu) and zinc (Zn) was examined. The quantity of total protein (mg protein/mg dry wt) in each sample and the measurement of MT were determined by the Lowry and silver (Ag)-saturation methods. Our results show that the gonads are the organs producing the greatest quantity of MT in the controls and in the groups induced by Cu and Zn, followed by the mantle, gut, and by muscle and plasma. Competition by Zn with respect to Cu for the production of MT has been established.

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References

  1. J. H. R. Kägi and A. Schäffer, Biochemistry of metallothionein,Biochemistry 27, 8508–8514 (1988).

    Google Scholar 

  2. B. Halliwell and J. M. C. Gutteridge, Protection against oxidants in biological systems: the superoxide theory of oxygen toxicity, inFree Radicals in Biology and Medicine, Clarendon, Oxford, pp. 130–133 (1989).

    Google Scholar 

  3. J. Abel and N. De Rutier, Inhibition of hidroxyl radical-generated DNA degradation by metallothionein,Toxicol. Lett. (Amst.) 47(2), 191–6 (1989).

    Article  CAS  Google Scholar 

  4. K. R. Etzel, M. R. Swerdel, J. N. Swerdel and R. J. Cousins, Endotoxin induced changes in copper and zinc metabolism in the syrian hamster,J. Nutr. 112, 2363–2373 (1982).

    PubMed  CAS  Google Scholar 

  5. D. H. Hamer, Metallothionein,Annu. Rev. Biochem. 55, 913–951 (1986).

    PubMed  CAS  Google Scholar 

  6. B. A. Fowler, C. E. Huldebrand, Y. Kojima and M. Webb,Experientia, Suppl. 52, 19–22 (1987).

    CAS  Google Scholar 

  7. D. R. Winge, K. B. Nielson, R. D. Zeikus and W. R. Gray, Structural Characterization of isoforms of prenatal and adult rat liver metallothionein,J. Biol. Chem. 29, 11419–11425 (1984).

    Google Scholar 

  8. C. T. Schmidt, M. F. Jubier and D. H. Hamer, Structure and expression of two human metallothionein-I isoform genes and a related pseudogene,J. Biol. Chem. 260, 7731–7737 (1985).

    PubMed  CAS  Google Scholar 

  9. C. D. Klaassen and L. D. Lehman-Mckeeman, Induction of metallothionein,J. Amer. College Toxicol. 8, 1315–1318 (1989).

    Google Scholar 

  10. I. Bremmer,Experientia Suppl. 52, 81–107 (1987).

    Google Scholar 

  11. B. A. Fowler, D. W. Engel and M. Mrower, Purification and characterization studies of cadmium-binding proteins from the american oysters, Crassostea virginica,Environ. Health Perspect. 15, 63–69 (1986).

    Article  Google Scholar 

  12. A. M. Scheuhammer and M. G. Cherian, Quantification of metallothionein by silver saturation,Methods Enzymol. 205, 78–83 (1991).

    Article  PubMed  CAS  Google Scholar 

  13. M. C. Torres and M. C. Martín Mateo,Abstr. Congress of the Mexican Biochemical Society, Ixtapa, México (1992).

  14. A. Moral and M. C. Martín Mateo,Abstr. 22th Meeting of the Federation of European Biochemical Societies, Stockholm, Sweden (1993).

  15. Y. Aoki, M. Kunimoto, Y. Shibata and K. Suzuki, Detection of metallothionein and nitrocellulose membrane using western blotting techniques and its aplication of cadmium-binding proteins,Anal. Biochem. 157, 117–122 (1989).

    Article  Google Scholar 

  16. J. Tsai, J. Murphy and P. K. Chien,Induction and Accumulation of Metallothionein in Different Organs of the Pacific Oyster (Crassostea gigas). Department of Biology, University of San Francisco, San Francisco (1990).

    Google Scholar 

  17. R. D. Barnes, Moluscos bivalvos, inZoologia de los Invertebrados, Ed. Interamericana, México, pp. 335–375 (1991).

    Google Scholar 

  18. M. Nordberg and Y. Kojima, Metallothionein and other low molecular weight metal binding proteins, inMetallothionein, J. H. R. Fägi and M. Nordberg, eds., Birkhauser Verlag, Basel, pp. 41–93 (1979).

    Google Scholar 

  19. S. Yu, Site of synthesis of metallothionein in rat liver,Can. J. Biochem. 59, 301–306 (1981).

    Article  PubMed  Google Scholar 

  20. J. H. R. Kägi and Y. Kojima,Experientia Suppl. 52, 25–61 (1987).

    Google Scholar 

  21. P. Oestreicher and R. J. Cousins, Influence of intraluminal constituents on zinc absorption by isolated vascularly perfused rat intestine,J. Nutr. 115, 159–166 (1985).

    PubMed  CAS  Google Scholar 

  22. A. C. Hall, B. W. Young and I. Bremmer, Intestinal metallothionein and the mutual antagonism between cooper and zinc in the rat.J. Inorg. Biochem. 11, 57–66 (1979).

    Article  PubMed  CAS  Google Scholar 

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Alonso, J.I., Martin-Mateo, M.C. Induction and characterization of metallothionein in different organs ofOstrea edulis L. . Biol Trace Elem Res 53, 85–94 (1996). https://doi.org/10.1007/BF02784547

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  • DOI: https://doi.org/10.1007/BF02784547

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