Abstract
To investigate the physiological roles or toxicity of trace or toxic elements, multielement analysis of limited quantities of samples in the biological tissues is required. Inductively coupled plasma mass spectrometry (ICP-MS) suits this requirement, but spectral and nonspectral interferences are inevitable. We examined correction methods for the nonspectral interferences by analyzing signals of 21 elements in various concentrations of HNO3 as well as five major elements (Na, K, P, Ca, and Cl). Using internal standards, the interferences caused by the major elements were corrected, but the interferences caused by HNO3 were impossible to correct for elements with high ionization potentials. The analytical results using the standard addition method on 14 elements in standard reference materials and fresh brain tissues confirmed the accuracy of this method. Thus, we concluded that the standard addition method is useful to correct for the nonspectral interferences.
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References
F.M. Corrigan, G. P. Reynolds, and N. I. Ward, Reduction of zinc and selenium in brain in Alzheimer’s disease,Trace Elements in Med. 8, 1–5 (1991).
R. Beliveau, Y. Gelinas, J. Ferraris, and J. P. Schmit, Complete analysis of the trace elements of the kidney,Biochem. Cell. Biol. 68, 1272–1280 (1990).
L. Moens, H. Vanhoe, J. Goossens, M. Campbell, and R. Dams, Application of inductively coupled plasma mass spectrometry to the certification of reference materials from the Community Bureau of Reference,J. Anal. At. Spectrom. 9, 187–191 (1994).
J. Goosens, F. Vanhaecke, L. Moens, and R. Dams, Elimination of interferences in the determination of arsenic and selenium in biological samples by inductively coupled plasma mass spectrometry,Analytica Chimica Acta 280, 137–143 (1993).
D. Beauchemin, J. W. McLaren, S. N. Willie, and S. S. Berman, Determination of trace metals in marine biological reference materials by inductively coupled plasma mass spectrometry,Anal. Chem. 60, 687–691 (1988).
C. Vandecasteele, H. Vanhoe, and R. Dams, Inductively coupled plasma mass spectrometry of biological samples,J. Anal. At. Spectrom. 8, 781–786 (1993).
S. H. Tan and G. Horlick, Background spectral features in inductively coupled plasma mass spectrometry,Appl. Spectroscopy 40, 445–460 (1986).
G. V. Iyengar, W. E. Kollmer, and H. J. M. Bowen, The elemental composition of human tissues and body fluids: A compilation of values for adults,Verlag Chemie, Weinheim 14 (1978).
A. Ejima, C. Watanabe, H. Koyama, and H. Satoh, Analysis of trace elements in the central nerve tissues with inductively coupled plasma-mass spectrometry,Tohoku J. Exp. Med. 178, 1–10 (1996).
R. Mizushima, M. Yonezawa, A. Ejima, H. Koyama, and H. Satoh, Microwave digestion in small amount biological samples,Tohoku J. Exp. Med. 178, 75–79 (1996).
K. Sumino, K. Hayakawa, T. Shibata, and S. Kitamura, Heavy metals in normal Japanese tissues,Arch. Environ. Health 30, 487–494 (1975).
N. A. Larsen, H. Pakkenberg, E. Damsgaard, and K. Heydorn, Topographical distribution of arsenic, manganese, and selenium in the normal human brain,J. Neurol. Sci. 42, 407–416 (1979).
W. R. Markesbery, W. D. Ehmann, M. Alauddin, and T. I. M. Hossain, Brain trace element concentrations in aging,Neurobiol. Aging 5, 19–28 (1984).
M. Gerotto, E. Dell’Andrea, A. Bortoli, M. Marchiori, M. Palonta, and A. Troncon, Interference effects and their control in ICP-MS analysis of serum and saline solutions,Microchem. J. 51, 73–87 (1995).
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Ejima, A., Watanabe, C., Koyama, H. et al. Matrix interferences in the analysis of digested biological tissues with inductively coupled plasma-mass spectrometry. Biol Trace Elem Res 69, 99–109 (1999). https://doi.org/10.1007/BF02783862
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DOI: https://doi.org/10.1007/BF02783862