Abstract
Iodide and acrylamide were applied simultaneously in a doublequenching experiment to compare acrylamide quenching constants for internal and external fluorophores of high-density lipoproteins (HDL1 and HDL2) from manganese-adequate (MnA) and deficient (MnD) rats, free of the electrostatic effects associated with iodide. In MnA HDL1 compared to MnD HDL1, the acrylamide quenching constant for external fluorophores was different (P < 0.1). In MnA HDL2, there were two populations of fluorophores accessible to acrylamide, whereas in MnD HDL2, all fluorophores were accessible to both quenchers. We concluded that there were structural (local environmental) differences, possibly charge-related, around the external fluorophores, and a slightly larger population of buried fluorophores in the MnD HDL1 compared with MnA HDL1. In MnA HDL2, one-third of the fluorophores were accessible to iodide, and all external and internal fluorophores were accessible to acrylamide, whereas in MnD HDL2, all fluorophores were accessible to both quenchers.
Similar content being viewed by others
References
J. Kawano, D. M. Ney, C. L. Keen, and B. O. Schneeman, Altered high density lipoprotein composition in manganese-deficient Sprague-Dawley and Wistar rats,J. Nutr. 117, 902–906 (1987).
C. D. Davis, D. M. Ney, and J. L. Greger, Manganese, iron and lipid interactions in rats,J. Nutr. 120, 507–513 (1990).
D. J. Klimis-Tavantzis, P. N. Taylor, R. A. Lewis, A. L. Flores, and H. H. Patterson, Effects of dietary manganese deficiency on high density lipoprotein composition and metabolism in Sprague-Dawley rats,Nutr. Res. 13, 953–968 (1993).
P. N. Taylor, H. H. Patterson, I. Wolinsky, and D. J. Klimis-Tavantzis, Manganese deficiency affects HDL1 and HDL2 composition in rats,Nutr. Res. 17, 1155–1162 (1997).
P. N. Taylor, H. H. Patterson, and D. J. Klimis-Tavantzis, Manganese deficiency alters high-density lipoprotein subclass structure in the sprague-dawley rat,J. Nutr. Biochem. 7, 392–396 (1996).
B. J. Friedman, J. H. Freeland-Graves, C. W. Bales, F. Behmardi, R. L. Shorey-Kutschke, R. A. Willis, J. B. Crosby, P. C. Trickett, and S. D. Houston, Manganese balance and clinical observations in young men fed a manganese-deficient diet,J. Nutr. 117, 133–143 (1987).
P. Johnson and G. I. Lykken, Manganese and calcium absorption and balance in young women fed diets with varying amounts of manganese and calcium,J. Trace Element Exp. Med. 4, 19–35 (1991).
Q. Q. Dang, P. Douste-Blazy, R. Camare, and D. Galy, Fluorescence quenching by iodide ions of low density lipoproteins from normolipidemic and hypercholesterolemic type Ha subjects. Effect of low density lipoprotein-cholesterol and low density lipoprotein non-apolipoprotein-B,Chem. Phys. Lipids 36, 121–130 (1984).
B. Somogyi, S. Papp, A. Rosenberg, I. Seres, J Matkó, G. R. Welch, and P. Nagy, A double-quenching method for studying protein dynamics: separation of the fluorescence quenching parameters characteristic of solvent-exposed and solvent-masked fluorophores,Biochemistry 24, 6674–6679 (1985).
M. R. Eftink and L. A. Selvidge, Fluorescence quenching of liver alcohol dehydrogenase by acrylamide,Biochemistry 21, 117–125 (1982).
K. A. Hageman and M. R. Eftink, Fluorescence quenching of Trp-314 of liver alcohol dehydrogenase by oxygen,Biophys. Chem. 20, 201–207 (1984).
P. G. Varley, D. T. F. Dryden, and R. H. Pain, Resolution of the fluorescence of the buried tryptophan in yeast 3-phosphoglycerate kinase using succinimide,Biochim. Biophys. Acta 1077, 19–24 (1991).
G. Gonzalez and G. Tapia, Fluorescence study of the thyroxine-dependent conformational changes in human serum transthyretin,FEBS Lett. 297, 253–256 (1992).
B. Somogyi and Z. Lakos, Protein dynamics and fluorescence quenching, /.Photochem. Photobiol. B: Biol. 18, 3–16 (1993).
G. L. Mills, P. A. Lane, and P. K. Weech, The collection and preservation of blood plasma, in A Guidebook to Lipoprotein Technique. Laboratory Techniques in Biochemistry and Molecular Biology, vol.14, R. H. Burdon and P. H. van Knippenberg, eds., Elsevier, New York, pp. 449–459 (1984).
D. J. Mela, R. S. Cohen, and P. M. Kris-Etherton, Lipoprotein metabolism in a rat model of diet-induced adiposity, /.Nutr. 117, 1655–1662, (1987).
B. G. Johansson, Agarose gel electrophoresis,Scand. Clin. Lab. Invest. 29 (Suppl. 124), 7–19 (1972).
J. Miller,Standards in Fluorescence Spectrometry, Chapman and Hall, London (1981).
B. C. MacDonald, S. J. Lvin, and H. H. Patterson,Anal. Chim. Acta 338, 155–162 (1997).
O. Stern and M. Volmer, über die Abklingungszeit der Fluoreszenz,Phys. Z. 20, 183–188 (1919).
J. R. Lakowicz,Principles of Fluorescence Spectroscopy, Plenum, New York (1983).
S. S. Lehrer, Solute perturbation of protein fluorescence. The quenching of the tryptophan fluorescence of model compounds and of lysozyme by iodide ion,Biochemistry 10, 3254–3263 (1971).
Y. Oschry and S. Eisenberg, Rat plasma lipoproteins: re-evaluation of a lipoprotein system in an animal devoid of cholesteryl ester transfer activity, /.Lipid Res. 23, 1099–1106 (1982).
S. Eisenberg, Plasma lipoprotein conversions,Methods Enzymol. 129, 347–366 (1986).
N. E. Miller, Associations of high-density lipoprotein subclasses and apolipoproteins with ischemic heart disease and coronary atherosclerosis,Am. Heart j. 113, 589–597 (1987).
D. J. Klimis-Tavantzis, P. N. Taylor, and I. Wolinsky, Manganese, lipid metabolism and atherosclerosis, inManganese in Health and Disease, D. J. Klimis-Tavantzis, ed., CRC, Boca Raton, FL, pp. 87–100 (1994).
P. Alaupovic, Significance of apolipoproteins for structure, function, and classification of plasma lipoproteins,Methods Enzymol. 263, 32–60 (1996).
G. Pifat, L. Udovicic, J. Brnjas-Kraljevic, G. Jürgens, A. Holasek, and J. N. Herak, Competitive ion binding to low density lipoproteins: an electron spin resonance study,Chem. Phys. Lipids 46, 99–105 (1988).
R. W. Mahley and T. L. Innerarity, Lipoprotein receptors and cholesterol homeostasis,Biochim. Biophys. Acta 737, 197–222 (1983).
R. Ekanayake, Ultrastructural evidence for adverse effects on aorta and liver in rats fed a short term manganese-deficient diet, MS thesis, University of Maine, Orono, ME, USA (1995).
P. Yang and D. Klimis-Tavantzis, Effect of dietary manganese on arterial glycosaminoglycan metabolism,FASEB J. 9, A576 (1995).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Taylor, P.N., Patterson, H.H. & Klimis-Tavantzis, D.J. A fluorescence double-quenching study of native lipoproteins in an animal model of manganese deficiency. Biol Trace Elem Res 60, 69–80 (1997). https://doi.org/10.1007/BF02783310
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02783310