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Membrane Effects of N-Terminal Fragment of Apolipoprotein A-I: A Fluorescent Probe Study

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Abstract

The binding of monomeric and aggregated variants of 1–83 N-terminal fragment of apolipoprotein A-I with substitution mutations G26R, G26R/W@8, G26R/W@50 and G26R/W@72 to the model lipid membranes composed of phosphatidylcholine and its mixture with cholesterol has been investigated using fluorescent probes pyrene and Laurdan. Examination of pyrene spectral behavior did not reveal any marked influence of apoA-I mutants on the hydrocarbon region of lipid bilayer. In contrast, probing the membrane effects by Laurdan revealed decrease in the probe generalized polarization in the presence of aggregated proteins. suggesting that oligomeric and fibrillar apoA-I species induce increase in hydration degree and reduction of lipid packing density in the membrane interfacial region. These findings may shed light on molecular details of amyloid cytotoxicity.

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Acknowledgments

This work was supported by the grant from Fundamental Research State Fund of Ukraine (project number F54.4/015) and Grant-in-Aid for Scientific Research 25293006 (to H.S.) from the Japan Society for the Promotion of Science.

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Authors and Affiliations

  1. Department of Nuclear and Medical Physics, V.N. Karazin Kharkiv National University, 4 Svobody Sq., Kharkov, 61077, Ukraine

    Valeriya Trusova, Galyna Gorbenko & Mykhailo Girych

  2. Institute of Health Biosciences, Graduate School of Pharmaceutical Sciences, The University of Tokushima, 1-78-1 Shomachi, Tokushima, 770-8505, Japan

    Emi Adachi, Chiharu Mizuguchi & Hiroyuki Saito

  3. Department of Biomedical Engineering and Computational Science, School of Science and Technology, Aalto University, 00076, Espoo, Finland

    Rohit Sood & Paavo Kinnunen

  4. 19-32 Geroyev Truda Str., Kharkov, 61144, Ukraine

    Valeriya Trusova

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  1. Valeriya Trusova
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  2. Galyna Gorbenko
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Correspondence to Valeriya Trusova.

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Trusova, V., Gorbenko, G., Girych, M. et al. Membrane Effects of N-Terminal Fragment of Apolipoprotein A-I: A Fluorescent Probe Study. J Fluoresc 25, 253–261 (2015). https://doi.org/10.1007/s10895-015-1501-9

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  • DOI: https://doi.org/10.1007/s10895-015-1501-9

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