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Gonadotrophin and prolactin secretion in castrated male sheep following subcutaneous or intracranial treatment with testicular hormones

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Abstract

Interactions between testosterone, estradiol, and inhibin in the control of gonadotrophin secretion in males are poorly understood. Castrated rams were treated with steroid-free bovine follicular fluid (bFF), testosterone, or estradiol and for 7 d(2×2×2 factorial design). Given independently, none of the exogenous hormones affected follicle-stimulating hormone (FSH) concentrations, but the combination of one or both steroids with bFF reduced FSH secretion. Testosterone and estradiol reduced luteinizing hormone (LH) pulse frequency (there was no synergism), and bFF had no effect. Plasma prolactin concentrations were not affected by any treatment. To locate the central sites of steroid action, castrated rams were bilaterally implanted in the preoptic area (POA), ventromedial nucleus (VMH), or arcuate nucleus (ARC). These implants did not affect FSH or prolactin concentrations, or LH pulse amplitude. The frequency of the LH pulses was not affected by testosterone in any site. Estradiol located in the ARC, but not the POA or VMH, decreased LH pulse frequency. In summary, FSH secretion is controlled by synergistic interactions between inhibin and estradiol or testosterone, whereas GnRH/LH pulse frequency is controlled by testicular steroids. Estradiol acts partly, at least, in the ARC, but the central site of action, testosterone remains unknown.

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References

  1. Martin, G. B. (1984).Biol. Rev.,59, 1–87.

    Article  PubMed  CAS  Google Scholar 

  2. Martin, G. B., Price, C. A., Thiéry, J.-C., and Webb, R. (1988).J. Reprod. Fertil. 82, 319–328.

    Article  PubMed  CAS  Google Scholar 

  3. Blache, D., Fabre-Nys, C., and Venier, G. (1991).Brain Res. 546, 241–249.

    Article  PubMed  CAS  Google Scholar 

  4. Blache, D., Fabre-Nys, C., and Venier, G. (1996).Brain Res. 741, 117–122.

    Article  PubMed  CAS  Google Scholar 

  5. Thiéry, J. C., Pelletier, J., and Signoret, J. P. (1978).Ann. Biol. Anim. Biochem. Biophys.,18, 1413–1426.

    Article  Google Scholar 

  6. Bolt, D. J. (1971).J. Reprod. Fertil.,24, 435–438.

    Article  PubMed  CAS  Google Scholar 

  7. Crim, L. W., and Geschwind, I. I. (1972).Biol. Reprod. 7, 47–54.

    PubMed  CAS  Google Scholar 

  8. Riggs, B. L. and Malven, P. V. (1974).J. Anim. Sci. 38, 1239–1244.

    PubMed  CAS  Google Scholar 

  9. Blanc, M. R., and Dacheux, J. L. (1976).IRCS Med. Sci. 4, 460.

    CAS  Google Scholar 

  10. Keogh, E. J., Lee, V. W. K., Rennie, G. C., Burger, H. G., Hudson, B., and De Krester, D. M. (1976).Endocrinology 98, 997–1004.

    PubMed  CAS  Google Scholar 

  11. Schanbacher, B. D. and Ford, J. J. (1977).Endocrinology 100, 387–393.

    PubMed  CAS  Google Scholar 

  12. Main, S. J., Davies, R. V., and Setchell, B. P. (1979).J. Reprod. Fertil. Suppl. 26, 3–14.

    Google Scholar 

  13. Parrott, R. T. and Davies, R. V. (1979).J. Reprod. Fertil. 56, 543–548.

    PubMed  CAS  Google Scholar 

  14. Schanbacher, B. D. (1979).J. Reprod. Fertil. Suppl. 26, 15–16.

    Google Scholar 

  15. D’Occhio, M. J., Schanbacher, B. D., and Kinder, J. E. (1983).Acta Encdocrinologica 102, 499–504.

    CAS  Google Scholar 

  16. Schanbacher, B. D. (1984).Endocrinology 115, 944–950.

    PubMed  CAS  Google Scholar 

  17. Pelletier, J. (1974).J. Reprod. Fertil. 41, 397–402.

    PubMed  CAS  Google Scholar 

  18. Galloway, D. B. and Pelletier, J. (1975).J. Endocrinol. 64, 7–16.

    PubMed  CAS  Google Scholar 

  19. Garnier, D. H., Terqui, M., and Pelletier, J. (1977).J. Reprod. Fertil. 49, 359–361.

    Article  PubMed  CAS  Google Scholar 

  20. Bremmer, W. J., Findlay, J. K., Lee, V. W. K., De Krestser, D. M., and Cumming, I. A. (1980).Endocrinology 106, 329–336.

    Google Scholar 

  21. D’Occhio, M. J., Schanbacher, B. D., and Kinder, J. E. (1982).Endocrinology 110, 1547–1554.

    PubMed  CAS  Google Scholar 

  22. Schanbacher, B. D. and D’Occhio, M. J. (1984).J. Reprod. Fertil. 72, 537–542.

    PubMed  CAS  Google Scholar 

  23. Tilbrook, A. J., DeKretser, D. M., Cummins, J. T., and Clarke, I. J. (1991).Endocrinology 129, 3080–3092.

    Article  PubMed  CAS  Google Scholar 

  24. Caraty, A., Bouchard, P., and Blanc, M. (1992). In: Modes of Action of GnRH and GnRH Analogs. Crowley, W. F. and Conn, P. M. (eds.) Springer-Verlag, Berlin, pp. 193–209.

    Google Scholar 

  25. Danguy, A., Ectors, F., Pasteels, J. L. (1977).J. Endocrinol. 73, 105–114.

    PubMed  CAS  Google Scholar 

  26. Herbison, A. E. (1995).J. Reprod. Fertil. Suppl. 49, 271–283.

    Google Scholar 

  27. Lehman, M. N. and Karsch, F. J. (1993).Endocrinology 133, 887–895.

    Article  PubMed  CAS  Google Scholar 

  28. Tilbrook, A. J., De Kretser, D. M., and Clarke, I. J. (1993).J. Endocrinol. 138, 181–189.

    Article  PubMed  CAS  Google Scholar 

  29. D’Occhio, M. J., Schanbacher, B. D., and Kinder, J. E. (1983).J. Androl. 4, 210–215.

    PubMed  CAS  Google Scholar 

  30. Schanbacher, B. D. and Ford, J. J. (1976).Endocrinology 99, 752–757.

    PubMed  CAS  Google Scholar 

  31. Buhl, A. E. and Cornette, J. C. (1982).Biol. Reprod. 26, 404–412.

    Article  PubMed  CAS  Google Scholar 

  32. Resko, J. A., Quadri, S. K., and Spies, H. G. (1977).Endocrinology 101, 215–224.

    PubMed  CAS  Google Scholar 

  33. Danguy, A., Pasteels, J. L., Ectors, F. (1977).J. Endocrinol. 74, 315–322.

    PubMed  CAS  Google Scholar 

  34. Lehman, M. N., Ebling, J. P., Moenter, S. M., and Karsch, F. J. (1993).Endocrinology 133, 876–886.

    Article  PubMed  CAS  Google Scholar 

  35. Blache, D., Batailler, M., and Fabre-Nys, C. (1994).J. Neuroendocrinol. 6, 329–339.

    Article  PubMed  CAS  Google Scholar 

  36. Kalra, P. S. and Kalra, S. P. (1980).Endocrinology 106, 390–397.

    PubMed  CAS  Google Scholar 

  37. Kalra, P. S. and Kalra, S. P. (1980).Endocrinology 111, 24–29.

    Google Scholar 

  38. Jackson, G. L., Kuehl, D., and Rhim, T. J. (1991).Biol. Reprod. 45, 188–194.

    Article  PubMed  CAS  Google Scholar 

  39. Tang, Y. P. and Sisk, C. L. (1988).J. Endocrinol. 117, 461–466.

    PubMed  CAS  Google Scholar 

  40. Verjans, H. L., Eik-Nes, K. B., Aafjes, J. H., Vels, F. J., and van der Molen, H. J. (1974).Acta Endocrinol. 77, 643–654.

    PubMed  CAS  Google Scholar 

  41. Tjondronegoro, S., Martin, G. B., Sutherland, S. R. D., and Boukhliq, R. (1996).Reprod. Fertil. Devel. 8, 855–862.

    Article  CAS  Google Scholar 

  42. Findlay, J. K., Robertson, D. M., Clarke, I. J., Klein, R., Doughton, B. W., Xiao, S., et al. (1992),Anim. Reprod. Sci. 28, 319–328.

    Article  CAS  Google Scholar 

  43. Lincoln, G. A. (1979).J. Endocrinol. 80, 133–140.

    PubMed  CAS  Google Scholar 

  44. Haug E. and Gautvik, K. M. (1976).Endocrinology 99, 1482–1489.

    PubMed  CAS  Google Scholar 

  45. Raymond, V., Beaulieu, M., Labrie, F., and Boissier, J. (1978).Science 200, 1173–1175.

    Article  PubMed  CAS  Google Scholar 

  46. Moore, K. E. (1987).Biol. Reprod. 36, 47–58.

    Article  PubMed  CAS  Google Scholar 

  47. Wallace, J. M. and McNeilly, A. S. (1985),J. Reprod. Fertil. 73, 505–515.

    Article  PubMed  CAS  Google Scholar 

  48. Knight, P. G., Wrathall, J. H. M., Glencross, R. G., and McLeod, B. J. (1991).J. Endocrinol. 128, 403–410.

    PubMed  CAS  Google Scholar 

  49. Fabre-Nys, C., Blache, D., Lavenet, C. (1991). In:Neuroendocrine Research Methods: Implantation and Transfection Procedures. Greenstein, B. D. (ed.) Harwood Academic Publishers: Chur, pp. 295–314.

    Google Scholar 

  50. Hötzel, M. J., Walkden-Brown, S. W., Blackberry M. A., and Martin, G. B. (1995).J. Endocrinol. 147, 75–85.

    PubMed  Google Scholar 

  51. Webb, R., Baxter, D., McBride, D., Nordblom, G. D., and Shaw, M. P. K. (1985).J. Steroid Biochem. 23, 1043–1051.

    Article  PubMed  CAS  Google Scholar 

  52. Merriam, G. R. and Wachter, K. W. (1982).Am. J. Physiol. 243, E310-E318.

    PubMed  CAS  Google Scholar 

  53. Steele, R. G. D. and Torrie, J. H. (1980).Principles and Procedures of Statistics. A Biometric Approach. McGraw-Hill, New York.

    Google Scholar 

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Authors and Affiliations

  1. Faculty of Agriculture (Animal Science), University of Western Australia, 6907, Nedlands, Australia

    Dominique Blache, Soedjiharti Tjondronegoro, Margaret A. Blackberry & Graeme B. Martin

  2. Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia

    Soedjiharti Tjondronegoro

  3. Department of Physiology and Pharmacology, University of Queensland, 4072, Queensland, Australia

    Stephen T. Anderson & Jonathan D. Curlewis

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  1. Dominique Blache
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  2. Soedjiharti Tjondronegoro
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  3. Margaret A. Blackberry
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  4. Stephen T. Anderson
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  5. Jonathan D. Curlewis
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  6. Graeme B. Martin
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Correspondence to Dominique Blache.

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Blache, D., Tjondronegoro, S., Blackberry, M.A. et al. Gonadotrophin and prolactin secretion in castrated male sheep following subcutaneous or intracranial treatment with testicular hormones. Endocr 7, 235–243 (1997). https://doi.org/10.1007/BF02778146

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  • DOI: https://doi.org/10.1007/BF02778146

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