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Effects of GH on IGF-II-induced progesterone accumulation by cultured porcine granulosa cells

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Abstract

A total of seven experiments were conducted to investigate the potential facilitative interaction of growth hormone (GH) and insulin-like growth factor II (IGF-II) in stimulating steroidogenesis by cultured porcine granulosa cells and to examine the possible nature of this action. Porcine granulosa cells were cultured in serum-free medium in the presence or absence of GH or prolactin, with or without IGF-II or IGF-I. IGF-II by itself dose (with peak progesterone production of 498 ng/mg DNA/24 h being observed at 100 ng of IGF-II/mL) and time- (with minimum time requirement of 24–48 h) dependently increased progesterone accumulation (P<0.01). Neither GH (dose range 0, 5, 10, 50, 100, and 150 ng/mL) nor prolactin (dose range 0, 0.01, 0.1, 1,5, 10 μg/mL) alone stimulated progesterone accumulation when compared with the control (P>0.05). However, in the presence of IGF-II, GH proved to be a potent amplifier of IGF-II in progesterone production (P<0.01) with a minimum GH time requirement of 24–48 h. In contrast, prolactin did not influence IGF-II-induced progesterone accumulation (P>0.05). An inhibitory study showed that the presence of cycloheximide (3 μg/mL) or actinomycin D (1 μg/mL) blocked both the stimulatory effect of IGF-II on progesterone accumulation and the amplification of GH on IGF-II induced production (P>0.01), suggesting GH amplification of IGF-II-induced progesterone accumulation is a process involving gene transcription and translation. Northern blot analysis further demonstrated that GH amplification of IGF-II-induced steroidogenesis can be attributed, at least partially, to enhanced IGF-II-induced cytochrome P450 cholesterol side-chain cleavage mRNA by GH.

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Authors and Affiliations

  1. Department of Animal Science, University of Saskatchewan, 57N 5B5, Saskatoon, Saskatchewan

    Yong Ping Xu & Phil A. Thacker

  2. Department of Obstetrics and Gynecology, University of Saskatchewan, 57N 5B5, Saskatoon, Saskatchewan

    Jorge Chedrese

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  1. Yong Ping Xu
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Xu, Y.P., Chedrese, J. & Thacker, P.A. Effects of GH on IGF-II-induced progesterone accumulation by cultured porcine granulosa cells. Endocr 7, 157–163 (1997). https://doi.org/10.1007/BF02778137

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  • DOI: https://doi.org/10.1007/BF02778137

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