Summary
The authors adapted microdialysis for the study of intrapancreatic exocrine proteins. Immunoreactive cationic and anionic trypsinogen were continuously measured after the insertion of one probe inside the pig pancreas and another placed under the peritoneum on the anterior surface of the gland. The concentration initially declined in the dialysate from the intrapancreatic probe, followed by an increasing tendency until 120 minutes after the insertion, after which a decline to a relative steady state after four to six hours was observed. A dialysate with a lower concentration of enzymes was obtained from the probe on the pancreatic surface. In order to study the turnover of an exogenously administered substance, a microdialysis probe was inserted into the pancreas and human recombinant pancreatic secretory trypsin inhibitor was injected into the pancreatic duct. The inhibitor was cleared from the pancreas with an intrapancreatic halflife of approximately 45 minutes. Membrane function, measured in vitro before and after use in vivo, was not significantly affected after seven hours in the pig pancreas.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Delgado JMR, DeFeudis FV, Roth RH, et al. Dialytrode for long term intracerebral perfusion in awake monkeys. Arch Int Pharmacodyn Ther 1972;198:9–21.
Ungerstedt U, Pycock C. Functional correlates of dopamine neurotransmission. Bull Scweiz Akad Med Wiss 1974;30:44–55.
Meirieu O, Pairet M, Sutra JF, et al. Local release of monoamines in the gastrointestinal tract: an in vivo study in rabbits. Life Sci 1986;38:827–834.
Yokel RA, Lidums V, McNamara PJ, et al. Aluminium distribution into brain and liver of rats and rabbits following intravenous aluminium lactate or citrate: a microdialysis study. Toxicol Appl Pharmacol 1991;107:153–163.
Van Wylen DGL, Willis J, Sodhi J, et al. Cardiac microdialysis to estimate interstitial adenosine and coronary blood flow. Am J Physiol 1990;258:H1842-H1649.
Ben-Nun J, Cooper RL, Cringle SJ, et al. Ocular dialysis. A new technique for in vivo intraocular pharmacokinetic measurements. Arch Ophthalmol 1988;106:254–259.
Jarry H, Dietrich M, Barthel A, et al. In vivo demonstration of a paracrine, inhibitory action of met-enkephalin on adrenomedullary catecholamine release in the rat. Endocrinology 1989;125: 624–629.
Arner P, Bolinder J, Eliasson A, et al. Microdialysis of adipose tissue and blood for in vivo lipolysis studies. Am J Physiol 1988; 255:E737-E742.
Lehman A. Effects of microdialysis-perfusion with anisoosmotic media on extracellular amino acids in the rat hippocampus and skeletal muscle. J Neurochem 1989;53:525–535.
Nordenvall M, Ulmsten U, Ungerstedt U. Influenece of progesterone on the sodium and potassium concentrations of rat uterine fluid investigated by microdialysis. Gynecol Obstet Invest 1989; 28:73–77.
Jarry H, Einspanier A, Kanngiesser L et al. Release and effects of oxytocin on estradiol and progesterone secretion in porcine corpora lutea as measured by an in vivo microdialysis system. Endocrinology 1990;126:2350–2358.
Stock S, Fastbom J, Björkstrand E, et al. Effects of oxytocin on in vivo release of insulin and glucagon studied by microdialysis in the rat pancreas and autoradiographic evidence for [3H]oxytocin binding sites within the islets of Langerhans. Regul Pept 1990; 30:1–13.
Ohlsson K. Acute pancreatitis. Biochemical, pathophysiological and therapeutics aspects. Acta Gastroenterol Belg 1988;51:3–12.
Ohlsson K, Eddeland A. Release of proteolytic enzymes in bile-induced pancreatitis in dogs. Gastroenterology 1975;69:668–675.
Kazal LA, Spicer DS, Brahinsky RA. Isolation of a crystalline trypsin inhibitor-anticoagulant protein from pancreas. J Am Chem Soc 1948;70:3034–3040.
Mancini G, Carbonara AO, Heremans JF. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry 1965;2:235–254.
Laurell C-B. Electroimmuno assay. Scand J Clin Lab Invest (Suppl 124) 1972;29:21–37.
KÄllén R, Borgström A, FÄit K. Pancreatic enzymes in serum and urine as indicators of pancreatic allograft rejection in the pig. Transplantation 1989;48:376–381.
Eddeland A, Ohlsson K. A radioimmunoassay for measurement of human pancreatic secretory trypsin inhibitor in different body fluids. Biol Chem Hoppe Seyler 1978;359:671–675.
Eddeland A, Ohlsson K. Purification and immunochemical quantitation of human pancreatic secretory trypsin inhibitor. Scand J Clin Lab Invest 1978;38:261–267.
Kendrick KM. Use of microdialysis in neuroendocrinology. Methods Enzymol 1988;168:182–205.
Benveniste H, Hüttemeier PC. Micodialysis-theory and application. Prog Neurobiol 1990;35:195–215.
Lönnroth P, Jansson P-A, Smith U. A microdialysis method allowing characterization of intercellular water space in humans. Am J Physiol 1987;253:E228-E231.
Marks WH, Ohlsson K. Elimination of pancreatic secretory trypsin inhibitor from the circulation. A study in man. Scand J Gastroenterol 1983;18:955–959.
Eddeland A, Ohlsson K. Studies on the pancreatic secretory trypsin inhibitor in plasma and its complex with trypsin in vivo and in vitro. Scand J Clin Lab Invest 1978;38:507–515.
Author information
Authors and Affiliations
Additional information
This work was supported by grants from the Swedish Medical Research Council (projects 03910 and 08715), the Swedish Cancer Society (project 1300), the Medical Faculty of the University of Lund, the Foundation of Malmö General Hospital against Cancer, the Foundation of Albert Pahlsson and the Foundation of Torsten and Ragnar Söderberg.
Rights and permissions
About this article
Cite this article
Jönsson, P., Borgström, A. & Ohlsson, K. Measurements of exocrine proteins in the pig pancreas using microdialysis. Gastroenterol Jpn 27, 529–535 (1992). https://doi.org/10.1007/BF02777790
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02777790