Summary
To elucidate peritoneal absorption of pancreatic enzymes, plasma levels of amylase, lipase, and trypsinogen were measured after the intraperitonel injection of 10 ml human pancreatic juice in dogs. Plasma pancreatic amylase, lipase, and trypsinogen were determined using the immunoassay specific to the corresponding human pancreatic enzyme to exclude cross-reaction with the endogenous enzyme activities of the canine plasma. Plasma immunoreactivity of human amylase persistently rose during 24 h after the injection, whereas elevation of plasma amylase enzyme activity become significant only at 24 h. Increase of plasma lipase was not remarkable. Significant increase of the immunoreactivity was observed only at 24 h but there was no significant increase of the enzyme activity during this period. Plasma trypsinogen immunoreactivity peaked at 2 h remained significantly elevated during 24 h. The transperitoneal absorption of pancreatic enzymes in dogs was confirmed using intraperitoneal injection of human pancreatic juice combined with immunoassay specific to human pancreatic enzymes.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Popper HL, Necheles H: Pathways of enzymes into the blood in acute damage of of the pancreas. Proc Soc Exp Biol Med 1940;43:220–222.
Howard JM, Smith AK, Peters JJ: Acute pancreatitis: pathways of enzymes into the blood. Surgery 1949;26:161–166.
Egdahl RH: Mechanism of blood enzyme changes following the production of experimental pancreatitis. Ann Surg 1958;148:389–400.
Waterman NG, Walsky RS: Transperitoneal absorption of amylase in acute experimental pancreatitis. Surg Gynecol Obstet 1970;131: 729–732.
Lange JF, Beyaert PJ, van Vugt H, et al: Pathways of enzyme transfer in sodium taurocholate-induced acute hemorrhagic pancreatitis. Digestion 1986;305:229–236.
Mayer AD, Airey M, Hodgson J, McMachon MJ: Enzyme transfer from pancreas to plasma during acute pancreatitis. The contribution of ascitic fluid and lymphatic drainage of the pancreas. Gut 1985;26:876–881.
Sugimoto Y, Hayakawa T, Kondo T, et al: Peritoneal absorption of pancreatic enzymes in bile-induced acute pancreatitis in dogs. J Gastroenterol Hepatol 1990;5:493–498.
Ceska M, Birath K, Brown B: A new and rapid method for the clinical determination of α-amylase activities in human serum and urine. Optical conditions. Clin Chim Acta 1969;26:437–444.
Shihabi ZK, Bishop C: Simplified turbidimetric assay for lipase activity. Clin Chem 1971;17:1150–1153.
Ogawa Y, Hayakawa T, Kondo T, et al: Diagnostic values of serum immunoreactive trypsin assay in pancreatic diseases using enzyme immunoassay. Jpn J Gastroenterol 1987;84:74–83. (in Japanese)
Hayakawa T, Sugimoto Y, Kondo T: Serum trypsin in acute experimental pancreatitis in dogs. Dig Dis Sci 1986;31:1133 (abstr).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hayakawa, T., Kondo, T., Shibata, T. et al. Peritoneal absorption of pancreatic enzymes in dogs. Gastroenterol Jpn 27, 230–233 (1992). https://doi.org/10.1007/BF02777727
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02777727