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Amplification and overexpression of the c-erbB-2 protooncogene in human gastric cancer

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Summary

The c-erbB-2 protooncogene encodes a possible growth factor receptor. This gene has been studied as to whether it can be regarded as a prognostic indicator in human breast carcinoma. As amplification and overexpression of the gene have been reported in several adenocarcinomas, 24 specimens of human gastric cancers were examined by immunohistochemical staining (24 cases), by Southern blotting (23/24) and by Northern blotting (16/24). Amplification of the gene was detected in two moderately differentiated tubular adenocarcinomas (8.7%), and overexpression of c-erbB-2 m-RNA was detected in three moderately differentiated tubular adenocarcinomas (18.8%). By immunohistochemical staining of paraffin-embedded tissues using a polyclonal antibody to c-erbB-2 gene products, the cell membrane was stained positively in three cases of gastric cancers which overexpressed c-erbB-2 mRNA. Peritoneal metastases were found in six gastric cancers, including two moderately differentiated tubular adenocarcinomas in which amplification of c-erbB-2 occurred. These results suggest that amplification and overexpression of c-erbB-2 may be correlated with metastases in differentiated adenocarcinoma of the stomach.

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Authors and Affiliations

  1. First Department of Surgery, Dokkyo University School of Medicine, 880 Kitakobayashi Mibumachi, Shimotsuga-gun, 321-02, Tochigi, Japan

    Kinro Sasaki M.D., Munenori Azuma & Shigemitsu Shida

  2. Department of Microbiology, School of Medicine, Chiba University, Chiba, Japan

    Yoshimi Tomita & Bunsiti Simizu

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  1. Kinro Sasaki M.D.
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  2. Yoshimi Tomita
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  3. Munenori Azuma
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  4. Shigemitsu Shida
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  5. Bunsiti Simizu
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Sasaki, K., Tomita, Y., Azuma, M. et al. Amplification and overexpression of the c-erbB-2 protooncogene in human gastric cancer. Gastroenterol Jpn 27, 172–178 (1992). https://doi.org/10.1007/BF02777719

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  • DOI: https://doi.org/10.1007/BF02777719

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