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A simple method for the quantitative determination of urinary hippuric acid excreted after administration of sodium benzoate for use as a liver function test

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Abstract

Improved and simpler method for the quantitative determination of hippuric acid in urine for the use as a liver function test has been presented. The acid was extracted from urine three times with mixture of ether and iso-propanol respectively, and hippuric acid was determined at 230 mμ. The results agree with the crystallization method of Krause, the colorimetric method using p-dimethylaminobenzaldehyde in acetic anhydrate, as well as with our colorimetric method using benzene sulfonyl chloride. It is also indicated that the colorimetric method using benzene sulfonyl chloride may be used as a more specific method for urinary hippuric acid assay.

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Authors and Affiliations

  1. Department of Public Health, Okayama University Medical School, Japan

    Masana Ogata & Yoshiko Takatsuka

  2. The First Department of Internal Medicine, Okayama University Medical School, Japan

    Tadasuke Kondo

Authors
  1. Masana Ogata
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  2. Yoshiko Takatsuka
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  3. Tadasuke Kondo
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Ogata, M., Takatsuka, Y. & Kondo, T. A simple method for the quantitative determination of urinary hippuric acid excreted after administration of sodium benzoate for use as a liver function test. Gastroenterol Jpn 6, 1–7 (1971). https://doi.org/10.1007/BF02776869

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  • DOI: https://doi.org/10.1007/BF02776869

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