Summary
The sulfokinase, that transfers activated sulfate from PAPS (3′-phosphoadenosine-5′phosphosulfate) to 4nitrocatechol sulfate was studied.
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1.
It is thought that the enzyme plays an important role in detoxication by the sulfation of polyhydric phenols in liver.
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2.
The enzyme had an optimum at pH 8.0 in Tris-acetate buffer. Km was 0.105 x 10-3M. The rate of conjugation was linear within 1 min.
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3.
The main distribution of the sulfokinase was found in the liver, lungs, spleen and various other organs in the rat. The activity of the enzyme in rat liver was the highest in soluble fraction. In chronic hepatic injury caused by carbon tetrachloride injection, the activity of the enzyme gradually decreased to below about onehalf of the control value.
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References
Nose Y, Lipmann F: Separation of steroid sulfokinase. J Biol Chem 233: 1348–1351, 1958
Giusti G, Piccinino F, Ricciardi I, Delrio G, Sagnelli E, Manzillo G: Abnormal steroid sulfate in plasma of women with intrahepatic cholestasis of pregnancy. Acta Hepatogastroenterol 26(3): 203–206, 1979
Rein G, Glover V, Sandler M: Sulphate conjugation of biologically active monoamines and their metabolites by human platelet phenolsulphotransferase. Clin Chim Acta 111: 247–256, 1981
Küchel O, Buu NN, Unger T, Lis M, Genest J: Fee and conjugated plasma and urinary dopamine in human hypertension. J Clin Endocrinol 48: 425–429, 1979
Yonei J: Studies on the excretion of direct bilirubins from the liver. Part 2. Difference of chemical properties of bilirubin sulfate isomers and their excretion into the rat bile. Jpn J Gastroenterol 76: 249–258, 1979
Du Duve C, Pressman BC, Gianetto R, Wattiaux R, Appelmans F: Tissue fraction studies VI. intracellular distribution patterns of enzyme in rat liver tissue. Biochem J 60: 604–617, 1955
Roy AB: The sulfation of ox liver I. the complex nature of the enzyme. Biochem J 53: 12–15, 1953
Lowry OH, Rosebrough NJ, Farr AJ, Randall RJ: Pro tein measurement with forin-phenol reagent. J Biol Chem 193: 265–275, 1951
Prockop DJ, Udenfriend S, Kari IK, Ossi LP: Modifi cations of a specific assay for hydroxyproline in urine. Anal Biochem 19: 249–255, 1967
Okuyama S: Analysis of free, glycine- and taurine-conjugated bile acids using highperformance liquid chromatography and immobilized 3. α-Hydroxy steroid dehydrogenase in column form. Rinsho byori 29: 446–458, 1981
Zimmerman L, Joernvall H, Bergstroem J, Fuerst P, Sjoevall J: Characterization of a double conjugate in uremic body fluids. Glucuronidated O-hydroxybenzoylglycine. Febs Lett 129(2): 237–240, 1981
Palmer RH: The formation of bile acid sulfate: a new pathway of bile acid metabolism in human. Proc Nat Acad Sci 58: 1047–1050, 1967
Adessi GL, Tran QN, Goutte CC, Jayle MF: In vitro demonstration of the hydrolysis of esterone and 17. Β-estradiol 3-sulfoconjugates by the uterus of pregnant guinea pigs. CR Seances Acad Sci, Ser 3, 292: 609–712, 1981
Axelson M, Sahlberg BL, Sjoevall J: Analysis of profiles of conjugated steroids in urine by ion-exchange separation and gas chromatography-mass spectrometry. J Chromatogr 224: 355–370, 1981
Ozeki T, Tokawa Y, Ogasawara T, Kubota Y, Takenaka K, Ito Y, Sato K, Kan M: Degradation of arylsulfatase by hepatic microsome. Clin Chem Acta 71:363–370, 1976
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Ozeki, T., Inoue, N., Kan, M. et al. A polyhydric phenol sulfokinase and chronic liver injury. Gastroenterol Jpn 17, 230–234 (1982). https://doi.org/10.1007/BF02776001
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DOI: https://doi.org/10.1007/BF02776001