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Hepatocyte plasma membrane antigens. II. Characterization of liver-specific membrane lipoprotein (LP-1) and tammhorsfall glycoprotein (THGP) like antigens (hepatic THGP) on the plasma membrane of chang liver cell

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Summary

Radioiodinated cell-surface antigens of Chang liver cells recognized with anti-liver-specific membrane lipoprotein (anti-LP-1) and anti-Tamm-Horsfall glycoprotein (anti-THGP) rabbit antibodies were analyzed by the immunoprecipitation followed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The analysis revealed that the antigens precipitated with anti-LP-1 were heterogeneous in molecular species consisting of 2 major and 5 minor polypeptides with molecular weights ranging from 36 × 103 (36 K) to 250 K daltons. That with anti-urinary THGP was a single polypeptide with a molecular weight of 67 K daltons, which was different from urinary THGP in molecular size, and was designated hepatic THGP.

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Authors and Affiliations

  1. First Department of Internal Medicine, Okayama University Medical School, 5-1, Shikatacho 2-chome, 700, Okayama, Japan

    Kohsaku Sakaguchi M.D., Norio Koide M.D., Hideaki Kondow M.D., Takayoshi Tanabe M.D., Michihiro Jitoku M.D., Terukatsu Arima M.D., Ph.D. & Hideo Nagashima M.D.

Authors
  1. Kohsaku Sakaguchi M.D.
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  2. Norio Koide M.D.
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  3. Hideaki Kondow M.D.
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  4. Takayoshi Tanabe M.D.
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  5. Michihiro Jitoku M.D.
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  6. Terukatsu Arima M.D., Ph.D.
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  7. Hideo Nagashima M.D.
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Additional information

This research was supported in a part by a research grant from the Scientific Research Fund (1982, No. 57770490) of the Ministry of Education, Science and Culture of Japan.

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Sakaguchi, K., Koide, N., Kondow, H. et al. Hepatocyte plasma membrane antigens. II. Characterization of liver-specific membrane lipoprotein (LP-1) and tammhorsfall glycoprotein (THGP) like antigens (hepatic THGP) on the plasma membrane of chang liver cell. Gastroenterol Jpn 18, 339–345 (1983). https://doi.org/10.1007/BF02774951

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  • DOI: https://doi.org/10.1007/BF02774951

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