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A specific gastrin receptor in human gastric mucosa

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Summary

Biological active125I-synthetic human gastrin (125I-SHG) appeared to bind specificially on human gastric mucosal membrane preparations. Gastrin binding was reversible, saturable and of high affinity (Kd=3.44 ±3.44 × 10-10M) with the binding site (3.66 ±0.34 fmol/mg protein) at 37°C for 30 min (pH 7.4). Specific gastrin binding was present in the fundic mucosa and absent from the antral, duodenal, jejunal, and colonic mucosa. Gastrin analogues (tetragastrin, pentagastrin, caerulein, and synthetic human gastrin) inhibited125I-SHG specific binding. However, the specific gastrin binding was not inhibited by glucagon, insulin, acetylcholine, atropine, histamine, or cimetidine. It was suggested that gastrin and histamine H2 receptor or gastrin and muscarinic cholinergic receptor did not share the same locus.

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References

  1. Code CF: Comparison of the effects of methantheline bromide (Banthin) and atropine on the secretory responses of vagally innervated and vagally denervated gastric pouches. Gastroenterology 19: 254, 1951

    PubMed  CAS  Google Scholar 

  2. Code CF: Histamine and gastric secretion, a later look, 1955–1965. Fed Proc 24: 1311, 1965

    PubMed  CAS  Google Scholar 

  3. Grossman MI, et al: Inhibition of acid secretion in dog by metiamide; a histamine antagonist acting on H2-receptors. Gastroenterology 66: 517, 1974

    PubMed  CAS  Google Scholar 

  4. Soll AH: The actions of secretagogues on oxygen uptake by isolated mammalian parietal cells. J Clin Invest 61: 370, 1978

    PubMed  CAS  Google Scholar 

  5. Soll AH: The interaction of histamine with gastrin and carbamylcholine on oxygen uptake by isolated mammalian parietal cells. J Clin Invest 61: 381, 1978

    Article  PubMed  CAS  Google Scholar 

  6. Soll AH, et al: Regulation of gastric acid secretion. Annu Rev Physiol 41: 35, 1979

    Article  PubMed  CAS  Google Scholar 

  7. Furchgott RF: Receptors. Fed Proc 37: 113, 1978

    PubMed  CAS  Google Scholar 

  8. Baur S, et al: A specific gastrin receptor on plasma membranes of antral smooth muscle. Biochem biophys Res Commun 73: 928, 1976

    Article  PubMed  CAS  Google Scholar 

  9. Lewin M, et al: Interaction of3H-labelled synthetic human gastrin I with rat gastric plasma membranes. Evidence for the existence of biologically reactive gastrin receptor sites. FFBS Lett 66: 168, 1976

    Article  CAS  Google Scholar 

  10. Lewin M, et al: Gastrin receptor sites in gastric mucosa, in “First International Symposium on Hormonal Receptors in Digestive Tract Physiology”, by Bonfils S, Fromageot P, Rosselin G. Elservier, Amsterdam, 1977, p 379

  11. Soumarman A, et al: Localization of gastrin receptors in intact isolated and separated rat fundic cells. Gastroenterology 73: 900, 1977

    Google Scholar 

  12. Brown J, et al: A specific gastrin receptor site in the stomach. Biochem Biophys Acta 538: 42, 1978

    PubMed  CAS  Google Scholar 

  13. Takeuchi K, et al: Mucosal gastrin receptor. I. Assay standardization and fulfillment of receptor criteria. Am J Physiol 237: E284, 1979

    PubMed  CAS  Google Scholar 

  14. Takeuchi K, et al: Mucosal gastrin receptor. II. Physical characteristics of binding. Am J Physiol 237: E295, 1979

    PubMed  CAS  Google Scholar 

  15. Takeuchi K, et al: Mucosal gastrin receptor. IV. Binding specificity. Am J Physiol 239: G395, 1980

    PubMed  CAS  Google Scholar 

  16. Plana RRV, et al: Effects of antigastrin drugs on the interaction of125I -human gastrin with rat gastric mucosa membranes. Hepato-gastroenterology 27: 41, 1980

    Google Scholar 

  17. Magous R, et al: High-affinity binding sites for gastrin on isolated rabbit gastric mucosal cells. Eur J Pharmac 82: 47, 1982

    Article  CAS  Google Scholar 

  18. Jacobs S, Cuatrecasas P: Problems in studying hormone receptor binding. 7th International Congress of Pharmacology. Abstracts, Pergamon Press Ltd, Paris, 1978, No 1340

    Google Scholar 

  19. Miyachi Y, et al: Enzymatic radioiodination of gonadotropins. J Clin Endocr 34: 23, 1972

    Article  PubMed  CAS  Google Scholar 

  20. Dockray GJ, et al: Biological activity of iodinated gastrins. Biochem Biophys Res Commun 69: 339, 1976

    Article  PubMed  CAS  Google Scholar 

  21. Ghosh MN, et al: Continuous recording of acid gastric secretion in the rat. Brit J Pharmacol 13: 54, 1958

    PubMed  CAS  Google Scholar 

  22. Lowry OH, et al: Protein measurement with the folin phenol reagent. J Biol Chem 193: 265, 1951

    PubMed  CAS  Google Scholar 

  23. Catt KJ, et al: Peptide hormone receptors. Ann Rev Physiol 39: 529, 1977

    Article  CAS  Google Scholar 

  24. Rosselin G, et al: Basal and hormone-stimulated adenylate cyclase in liver plasma membranes measurement by radioimmunoassay of cyclic AMP. Biochem Biophys Acta 304: 541, 1973

    PubMed  CAS  Google Scholar 

  25. Scatchard G: The attraction of proteins for small molecules and ions. Ann N Y Acad Sci 51: 660, 1949

    Article  CAS  Google Scholar 

  26. Konturex SJ, et al: Effect of medical and surgical vagotomy on gastric response to graded doses of pentagastrin and histamine. Gastroenterology 54: 392, 1968

    Google Scholar 

  27. Gibson R, et al: Actions of metiamide, an H2-histamine receptor antagonist, on gastric H+ and pepsin secretion in dogs. Gastroenterology 67: 93, 1974

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Second Department of Surgery, Kumamoto University Medical School, Honjo 1-1-1, 860, Kumamoto, Japan

    Takao Tokunaga M.D., Atsunobu Misumi M.D. & Masanobu Akagi M.D.

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  1. Takao Tokunaga M.D.
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  2. Atsunobu Misumi M.D.
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  3. Masanobu Akagi M.D.
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Tokunaga, T., Misumi, A. & Akagi, M. A specific gastrin receptor in human gastric mucosa. Gastroenterol Jpn 19, 1–8 (1984). https://doi.org/10.1007/BF02774640

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  • DOI: https://doi.org/10.1007/BF02774640

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