Summary
Liver cell damage is inducible when isolated liver cells coated with the specific antibody against the liver cell membrane are cultured with peripheral blood mononuclear cells. Moreover, a cytotoxic material (or materials) causing an inhibition of protein synthesis in liver cells was detected in culture supernatant of these antibody-dependent cell-mediated cytotoxicity (ADCC) reactions. By gel filtration using a Sephadex G-75 column, the active material (materials) was recovered in a definitive fraction and was further fractionated into two portions by DEAE-cellulose column chromatography. These active principles were shown by isoelectric focusing electrophoresis to be different substances from each other. These results suggest that some cytotoxic or cytostatic materials are produced in consequence of ADCC reaction and these may cause liver injury.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Meyer zum Büschenfelde KH, et al: Experimental chronic active hepatitis in rabbits following immunization with human liver proteins. Clin Exp Immunol 11: 99, 1972
Miller J, et al: Cell-mediated immunity to a human liver-specific antigen in patients with active chronic hepatitis and primary biliary cirrhosis. Lancet II: 296, 1972
Cochrane AM, et al: Antibody-dependent cell-mediated (K-cell) cytotoxicity against isolated hepatocytes in chronic active hepatitis. Lancet I: 441, 1976
Vogten AJM, et al: Cell-mediated cytotoxicity in chronic active liver disease. A new test system. Gastroenterology 74: 883, 1978
Meyer zum Büschenfelds KH, Miescher PA: Liver specific antigen — Purification and characterization. Clin Exp Immunol 10: 89, 1972
Bellemann P, et al: An improved method for the isolated of hepatocytes from liver slice. Selective removed of trypan blue-dyeable cells. Analytical Biochem 81: 408, 1977
Hopf U, Meyer zum Büschenfelds KH: Studies on the pathogenesis of experimental chronic active hepatitis in rabbits. II Demonstration of immunoglobulin on isolated hepatocytes. Br J Exp Pathol 55: 509, 1974
Granger GA, et al: Cytotoxic and growth inhibitory lymphokines. In: Biology of the lymphokines, Edited by Cohen S, Pick E, Oppenheim JJ. New York, Academic Press, 1978, pp 141–163
Yano K: Mechanisms of antibody dependent cell-mediated cytotoxicity (ADCC). Clinical Immunology (Jpn) 13: 116, 1981
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mizoguchi, Y., Monna, T., Yamamoto, S. et al. Studies on the mechanism of liver injury by antibody-dependent cell-mediated cytotoxicity (ADCC)—partial purification of cytotoxic factor detected in the culture supernatant of ADCC reaction. Gastroenterol Jpn 17, 360–367 (1982). https://doi.org/10.1007/BF02774583
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02774583