Summary
Two cases of macro-molecular GOT (glutamic oxaloacetic transaminase) were encountered recently. Both were middle-aged women, showing no abnormality in laboratory test results including those for GPT (glutamic pyruvic transaminase), except that abnormally high values (ca. 200 Karmen unit) were obtained for GOT. Clinical examinations and tests on the liver, heart, skeletal muscle and other organs were negative. By column chromatography, immunoelectrophoresis and other procedures, this macro-molecular GOT was shown to be an anzyme immunoglobulin complex with a molecular weight of about 250,000 formed by the binding of the GOT of the cytoplasmic fraction to Ig-G-Kappa.
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References
Wilding P, Cooke WT, Nicholson GI: Globulinbound amylase. A cause of persistently elevated levels in serum. Ann Int Med 60: 1053, 1964
Ganrot PO: Lupoid cirrhosis with serum lactic acid dehydrogenase linked to an γ A immunoglobulin. Experientia 23: 593, 1967
Nagamine M, Ohkuma S: Serum alkaline phosphatase isoenzymes linked to immunoglobulin G. Clin Chim Acta 65: 39, 1975
Kajita Y, Naito M, Shiozu N, et al: Antibody for glutamic pyruvic transaminase in chronic liver disease. Igaku no Ayumi 101: 652, 1977. (Jpn)
Iida K, Hayakawa M, Yamashita K, et al: GOT in the macro-molecular fraction. Rinsho-Byori (J Clin Pathol) 21 (suppl) 167, 1973. (Jpn)
Kuwa K, Nakayama T, Kitamura M: Determination of GOT, GPT and LDH by UV method using Hitachi M400. Proc Jpn Soc Clin Lab Automation 2: 55, 1977. (Jpn)
Boyd JW: The extraction and purification of two isoenzymes of L-Aspartate: 2-Oxoglutarate Aminotransferase. Biochim Biophys Acta 113: 302, 1966
Schmidt DM, Black MB, Miller H, et al: Modified microzone cellulose acetate method for lactic dehydrogenase isoenzymes — Normal values. Am J Med Technol35: 153, 1969
Scheidegger JJ: Une micro-méthode de l’immuno-élec-trophorèse. Int Arch Allergy 7: 103, 1955
Harada K, Nakayama T, Kitamura M, et al: Immunological and electrophoretical approaches to macro amylase analysis. Clin Chim Acta 59: 291, 1975
Kuwa K, Nakayama T, Kitamura M: A study on the affinity of immunoglobulins from LDH anomaly for five human LDH isoenzymes. Seibutsu-Butsuri-Kagaku (Phys Chem Biol)21: 209, 1977. (Jpn)
Kanno T: Enzyme-linked immunoglobulins. Rinsho-Kensa(J Medical Technology) 21: 381, 1977. (Jpn)
Kano S, Takeshita E, Kanno T: On the properties of so-called macro-alkaline phosphatase observed in ulcerative colitis — Alkaline phosphatase bonding immunoglobulin— Seibutsu-Butsuri-Kagaku (Phys Chem Biol) 20: 325, 1977. (Jpn)
Nagamine M: Lactate dehydrogenase isoenzymes linked to immunoglobulin A in two cases. Clin Chim Acta 36: 139, 1972
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Nakajima, M., Ito, K., Kuwa, K. et al. Occurrence of persistent elevated levels of serum glutamic oxaloacetic transaminase as a result of enzyme-immunoglobulin complex formation with a report of two cases. Gastroenterol Jpn 15, 330–336 (1980). https://doi.org/10.1007/BF02774303
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DOI: https://doi.org/10.1007/BF02774303