Summary
Using the simple thin layer polyacrylamide gel electrophoresis, serum alkaline phosphatase could be separated 5 isozyme bands in various digestive diseases, consisting of 54 cases of gastric cancer, 11 of colonie cancer, 12 of hepatoma, 4 of cholangioma, 14 of pancreatic cancer, 81 of benign hepatobilliary diseases, 13 of cancers of other organs and 61 of control. The obtained results were as follows:
-
1)
The electrophoretic analysis of serum alkaline phosphatase showed the specific band remaining at the origin, already reported as “alkaline phosphatase O“, in primary and metastatic cancer of the liver and cholelithiasis. On the contrary, alkaline phosphatase O was never found in gastric and colonie cancer without cancerous metastasis to the liver, and it was also inclined to be positive with the progress of liver metastasis among them.
-
2)
Intestinal alkaline phosphatase was usually found in higher frequency in blood group B and O than in the others, and it was apt to disappear in gastric or colonie cancer with an exacerbation of its cancerous lesions.
-
3)
Heat-stable alkaline phosphatase was found in 10% of gastric or colonie cancer, all of which were histologically proved to be well differentiated adenocarcinoma.
Similar content being viewed by others
References
Marken CL, M011er F: Multiple Forms Enzyms: Tissue, ontogenic and species specific patterns. Proc Nat Acad Sci Wash 45: 753–763, 1959
Fishman WH, Inglis NI, Stolbach LL, Krant MJ: A serum alkaline phosphatase Isozyme of human neoplastic cell origin. Cancer Res 28: 150–154, 1968
Fishman WH, Inglis NR, Green S, Anstiss CL, Gosh NK, Reif AE, Rustigian R, Krant MJ, Stolbach LL: Immunology and biochemistry of Regan isoenzyme of alkaline phosphatase in human cancer. Nature 219: 697–699, 1968
Nakayama T, Yoshida M, Kitamura M: L-leucin sensitive, heat-stable alkaline-phosphatase isozyme detected in a patient with pleuritis carcinomatosa. Clin chim Acta 30: 546–548, 1970
Boyer SH: Alkaline phosphatase in human sera and placentae. Svirnvr 134: 1002–1004, 1961
Inglis NR, Kirling S, Stolbach LL, Fishman WH: phenotypes of Regan isoenzyme and identity between the placental D-variant and the Nagao isoenzyme. Cancer Res 33: 1657–1661, 1973
Hattori N, Murayama H, Mitsui H, Arima M: High molecular alkaline phosphatase. Acta Hepatol Japan 10: 40–44, 1969 (in Japanese)
Dunne J, Fennelly JJ, MacGeeney K: Separation of alkaline phosphatase enzyme in human serum using gel-filtration (Sephadex G-200) techniques. Cancer 20: 71–76, 1967
Shinkai K, Akedo H: A multienzyme complex in serum of hepatic cancer. Cancer Res 32: 2307–2313
Warnock ML, Reisman R: Variant alkaline phosphatase in human hepatocellular cancers. Clin Chim Acta 40: 67–81, 1972
Higashino K, Hashinotsume M, Kang KY, Takahashi Y, Yamamura Y: Studies on a variant alkaline phosphatase in sera of patients with hepatocellular carcinoma. Clin Chim Acta 40: 67–81, 1972
Miki K, Suzuki H, Iino S, Niwa H, Oda T: Studies on alkaline phosphatase isoenzymes in carcinoma tissue of the stomach. Japan J Gastroenterol 73: 1527–1536, 1976 (in Japanese)
Miki K, Oda T, Suzuki H, Lino S, Niwa H: alkaline phosphatase isoenzymes in intestinal metaplasia and carcinoma of the stomach. Cancer Res 36: 4266–4268
Ogita Z, Sakoyama Y, Chiba Y, Masuzawa M: Separation of serum proteins by using a simple thin layer polyacrylamide gel electrophorsis. Physicochem.bioll 7: 71–76, 1973 (in Japanese)
Masuzawa M, Kamata T, Sakoyama Y, Chiba Y, Ogita Z: Studies on alkaline phosphatase isozymes in human serum by using a simple thin layer polyacrylamide gel electrophoresis. Physico-chem biol 17: 71–76, 1973
Japanese Research Society for Gastric cancer: The General Rules The Gastric Cancer Study In Surgery And Pathology. 9th ed 1974
Kawai K, Ida K, Kohli Y: On the dye scattering method for endoscopy, “New fields in gastrointestinal endoscopy-Urgent endoscopy of digestive and abdominal diseases, Int Symp Praquc/Carlsbad 1971, 257–260 (Karger, Basel, 1972)
Ida K, Kohli Y, Shimamoto K, Hashimoto K, Hashimoto Y, Kawai K: Endoscopical findings of fundic and pyloric gland area using dye scattering method. Endoscopy 5: 21–26, 1973
Hashimoto Y, Takeda S, Kohli Y, Ida Y, Kawai K: Dye scattering method in endoscopy for diagnosing early gastric cancer: Stomach and intestine 10: 1157–1164, 1975 (in Japanese)
Arfors KE, Beckman L, Lundin LG: Genetic variations of human serum phosphatase. Acta genet, Basel 13: 89–94, 1963
Yamasawa K: Studies on human alkaline phosphatase types in Japanese. Jap J Legal med 21: 552–557, 1964
Inglis NI, Krant MJ, Fishman WH: Influence of a fatenriched meal on serum (L-phenyl alanine-sensitive) “intestinal” alkaline phosphatase. Proc Soc Exp biol Med 124: 699–702, 1967
Stolbach LL, Krant MJ, Fishman WH: Ectopic production of an alkaline phosphatase isoenzyme in patients with cancer. New Engl J Med 281: 757–762, 1969
Nathanson L, Fishman WH: New observations on the Regan isoenzyme of alkaline phosphatase in cancer patients. Cancer 27: 1388–1397, 1971
Suzuki H, Lino S, Oda T: Heat-stable alkaline phosphatase in cancers. Int Med 29: 838–846, 1972 (in Japanese)
Higashino K, Hashinotsume M, Kudo S, Ohtani R, Hada T, Kyo K, Okohchi T, Takahashi Y, Ninaka T: Cancer, especially hepatoma, and alkaline phosphatase. Jap J clin Med 32: 1244-1257 (in Japanese)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yamaguchi, K., Fujimoto, S., Misaki, F. et al. Serum alkaline phosphatase (Al-pase) isozyme in gastric and colonic cancer. Gastroenterol Jpn 13, 264–271 (1978). https://doi.org/10.1007/BF02774049
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02774049