Summary
Serum Deoxyribonuclease (DNase) of normal persons and of patients with chronic pancreatitis, pancreatic cancer, Diabetes Mellitus, or other malignant diseases was determined with (32P) DNA as substrate. Serum DNase activity was much lower in patients with chronic pancreatitis, pancreatic cancer, or other malignant diseases than in control subjects, and serum DNase activity was almost normal in patients with Diabetes Mellitus.
There was no correlation between serum DNase and serum amylase, but there was a good correlation between serum DNase and DNase I output in duodenal juice. There was an inverse correlation between serum DNase and serum RNase.
These results imply that in the diagnosis of possible pancreatic disorders serum DNase may be a good indicator and thus may be useful for the detection of malignant diseases.
Similar content being viewed by others
References
Sun DCH, et al: Pancreozymin-Secretin test. Gastroenterology 38: 570, 1960
Sun DCH: Normal values for pancreozymin-secretin test. Gastroenterology 44: 602, 1963
Funakoshi A, et al: Analysis of serum deoxyribonuclease activity in comparison with normal and after endoscopic retrograde pancreatography. Gastroenterol Jpn (in press)
Funakoshi A, et al: Clinical studies on human pancreatic deoxyribonuclease I. Gastroenterol Jpn 14: 48, 1979
Funakoshi A, et al: Purification and properties of human pancreatic Deoxyribonuclease I. J Biochem 82: 1771, 1977
Anai M, et al: A deoxyribonuclease which requires nucleoside triphosphate fromMicrococcus lysodeikticus. J Biol Chem 245: 767, 1970
Funakoshi A, et al: Serum ribonucleases in pancreatic cancer: Relation to tumor histology. Gastroenterol Jpn 13: 390, 1978
Somogyi M: Micromethods for the estimation of diastase. J Biol Chem 125: 399, 1969
Kowlessar OD, et al: Deoxyribonuclease I activity in pancreatic disease. J Clin Invest 35: 1325, 1958
Dabrowska W, et al: A specific inhibitor for desoxyribonuclease. J Biol Chem 177: 991, 1950
Cooper EJ, et al: Occurence and distribution of an inhibitor for desoxyribonuclease in animal tissues. Proc Soc Exp Med 73: 219, 1949
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Funakoshi, A., Wakasugi, H. & Ibayashi, H. Clinical investigation of serum deoxyribonuclease: II. Clinical studies of serum deoxyribonuclease activity in pancreatic disease. Gastroenterol Jpn 14, 436–440 (1979). https://doi.org/10.1007/BF02773731
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02773731