Summary
Serum Deoxyribonuclease (DNase) micro-assay method was developed using32P-labelled E. coli DNA as substrate.
The serum DNase showed maximum activity at pH 7.5. It required Mg++ for activity, and was inhibited by EDTA or EGTA. The enzyme was also inhibited by actin (60-65%) or bovine pancreatic DNase I antibody (40-45%). The serum DNase activity was markedly increased following endoscopic retrograde pancreatography (ERP) examination. These results imply that serum DNase activity is mostly at least 60–65% pancreatic DNase I.
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Funakoshi, A., Kimura, T., Wakasugi, H. et al. Clinical investigation of serum deoxyribonuclease: I. Analysis of serum deoxyribonuclease activity in comparison with normal and after endoscopic retrograde pancreatography. Gastroenterol Jpn 14, 432–435 (1979). https://doi.org/10.1007/BF02773730
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DOI: https://doi.org/10.1007/BF02773730