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Evaluation of genetic diversity and germ plasm identification of 44 species, clones, and cultivars from 5 sections of the genusPopulus based on amplified fragment length polymorphism analysis

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Abstract

The genusPopulus L. (Salicaceae) can be divided into 5 sections with distribution throughout the world. Accurate identification ofPopulus clones and species is essential for effective selection, breeding, and management of genetic resources. In this study, amplified fragment length polymorphism (AFLP) analysis, which was reported as a reliable technique with high efficiency in detecting polymorphism, was used to conduct analyses of genetic diversity and variety identification of 44 species, clones, and cultivars ofPopulus that represent a wide range of breeding and commercially available germplasms. Cluster analysis of the 44 samples was carried out, and a dendrogram of genetic relatedness was developed on the basis of the AFLP data. DNA fingerprints of the 44 samples were developed from 12 selected bands amplified with 2 primer combinations (M-CAG/E-TA and M-CAG/E-TC). Each sample has its unique fingerprint pattern and can be distinguished from the others. Furthermore, 1 specific AFLP band of the cultivarPopulus canadensis cl. Guariento coming from fragments amplified by primer combination M-CTC/E-AG was successfully converted into a sequence-characterized amplified region (SCAR) marker. The results indicate that AFLP analysis should be considered as the preferred technique for the study of polymorphism inPopulus. This research is the first report concerning the use of AFLP analysis in genetic diversity and germplasm identification among all sections ofPopulus.

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Abbreviations

AFLP:

amplified fragment length polymorphism

CTAB:

cetyltrimethylammonium bromide

EDTA:

ethylene diamine tetraacetic acid

MAS:

marker-assisted selection

PAGE:

polyacrylamide gel electrophoresis

RAPD:

random-amplified polymorphic DNA

RFLP:

restriction fragment length polymorphism

SCAR:

sequence-characterized amplified region

SSR:

simple sequence repeat

UPGMA:

unweighted pair group method using arithmetic means

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Authors and Affiliations

  1. State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China

    Chun Jiang Zhou, Jian Wei Sun, Min De Jin & Bin Wang

  2. Graduate School of the Chinese Academy of Sciences, 100039, Beijing, China

    Chun Jiang Zhou

  3. Research Institute of Forestry, Chinese Academy of Forestry, 100091, Beijing, China

    Hong Zhu Song, Jin Hua Li & Qi Wen Zhang

Authors
  1. Chun Jiang Zhou
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  2. Hong Zhu Song
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  3. Jin Hua Li
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  4. Jian Wei Sun
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  5. Min De Jin
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  6. Qi Wen Zhang
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  7. Bin Wang
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Zhou, C.J., Zhu Song, H., Hua Li, J. et al. Evaluation of genetic diversity and germ plasm identification of 44 species, clones, and cultivars from 5 sections of the genusPopulus based on amplified fragment length polymorphism analysis. Plant Mol Biol Rep 23, 39–51 (2005). https://doi.org/10.1007/BF02772645

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