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A simple method forIn Situ hybridization to RNA in guard cells ofVicia Faba L.: The expression of aquaporins in guard cells

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Abstract

Because the epidermis ofV. faba L. leaves easily can be peeled into strips of one cell layer, we developed a simple method ofin situ hybridization using epidermal peels as a substitute for paraffin, resin and cryosections. Our method sufficiently detected the expression of broad bean aquaporin 1 in guard cells. RT-PCR revealed higher expression of aquaporins (AQPs) in guard cells compared to other leaf cell types; this indicates the importance of AQP for bulk water flow across guard cell membranes and, therefore, for stomatal movements.

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Abbreviations

Advin-AP:

advin-alkaline phosphatase

AQP:

aquaporin

BCIP:

5-bromo-4-chloro-3-indolyl-phosphate

DEPC:

diethlypyrocarbonate

GUS:

beta-glucuronidase

NBT:

nitro blue tetrazolium chloride

MIP:

major intrinsic protein

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Authors and Affiliations

  1. Institute of Plant Physiology, Shanghai Institutes for Biological Sciences/SIBS, Chinese Academy of Sciences/CAS, P. R. China

    Mei-Hao Sun, Wen Xu, Ya-Fang Zhu, Wei-Ai Su & Zhang-Cheng Tang

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  1. Mei-Hao Sun
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  2. Wen Xu
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  3. Ya-Fang Zhu
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  4. Wei-Ai Su
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  5. Zhang-Cheng Tang
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Sun, MH., Xu, W., Zhu, YF. et al. A simple method forIn Situ hybridization to RNA in guard cells ofVicia Faba L.: The expression of aquaporins in guard cells. Plant Mol Biol Rep 19, 129–135 (2001). https://doi.org/10.1007/BF02772155

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  • DOI: https://doi.org/10.1007/BF02772155

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