Skip to main content
SpringerLink
Log in

N-acetyl-B-D-glucosaminidase isoenzymes in the diagnosis of poisoning and kidney diseases

  • Published:
International Urology and Nephrology Aims and scope Submit manuscript

Abstract

Damaged lysosomes from renal tubular cells are, to the greatest degree, the source of activity of NAG in urine. Besides this, the enzyme can appear as a result of degranulation of granulocytes (PMN), active infection of the urinary system as also from serum as a result of glomerular filtration during damage to the glomerular basement membrane.

For the purpose of explaining the source of origin of the enzyme in urine, NAG was separated into isoenzymes from the kidneys, granulocytes and serum for comparison with isoenzymes in physiological and pathological urines after ethylene glycol poisoning, and glomerulonephritis, respectively.

The separation was made by column ion-exchange chromatography on DEAE-52 cellulose and by electrophoresis in 7% polyacrylamide gel. In addition, the thermostability of isolated isoenzymes was compared.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bourbouze, R., Raffi, F., Dameron, G., Hali-Miraftab, H., Loko, F., Vilde, J.-L.: N-acetyl-β-D-glucosaminidase (NAG) isoenzyme release from human monocytederived macrophages in response to zymozan and human recombinant interferon-γ.Clin. Chim. Acta, 199, 185 (1991).

    Article  CAS  PubMed  Google Scholar 

  2. Böyum, J.: Isolation of mononuclear cells and granulocytes from human blood. Isolation of mononuclear cells by one centrifugation and of granulocytes by combining centrifugation and sedimentation at 1×g.Scand. J. Clin. Lab. Invest. (Suppl.),97, 77 (1968).

    Google Scholar 

  3. Coward, R. A., DeLamore, I. W., Mallick, N. P., Robinson, L. E.: Urinary N-acetyl-β-D-glucosaminidase as an indicator of tubular damage in multiple myeloma.Clin. Chim. Acta, 138, 293 (1984).

    Article  CAS  PubMed  Google Scholar 

  4. Dance, N., Price, R. G., Cattel, W. R., Lansdell, Richards B: The excretion of N-acetyl-β-glucosaminidase and β-galactosidase by patients with renal disease.Clin. Chim. Acta, 27, 87 (1970).

    Article  CAS  PubMed  Google Scholar 

  5. Davis, B. J.: Disc electrophoresis. II. Method and application to human serum proteins.Ann. N.Y. Acad. Sci., 121, 404 (1964).

    Article  CAS  PubMed  Google Scholar 

  6. Ellis, B. G., Tucker, S. M., Thompson, A. E., Price, R. G.: Presence of serum and tissue forms of N-acetyl-β-glucosaminidase in urine from patients with renal disease.Clin. Chim. Acta, 64, 195 (1975).

    Article  CAS  PubMed  Google Scholar 

  7. Kind, P. R. N.: N-acetyl-β-D-glucosaminidase in urine of patients with renal disease, and after renal transplants and surgery.Clin. Chim. Acta, 119, 89 (1982).

    Article  CAS  PubMed  Google Scholar 

  8. Leaback, D. H., Walker, P. G.: The fluorimetric assay of N-acetyl-β-glucosaminidase.Biochem. J., 78, 151 (1961).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Simon, G., Morioka, S., Snyder, D. K.: Increased serum and urinary N-acetyl-β-D-glucosaminidase activity in human hypertension: Early indicator of renal dysfunction.Clin. Exp. Hyper. (Theor. Pract.),A6, 879 (1984).

    Article  CAS  Google Scholar 

  10. Synamiotis, C. A., Kaukautsakis, P., Spyridis, P.: Estimation of urinary N-acetyl-β-D-glucosaminidase activity for monitoring therapy of distal renal tubular acidosis.Acta Paediatr. Scand., 78, 453 (1989).

    Article  Google Scholar 

  11. Śkrha, J., Marek, J., Śrámková, J., Śtolba, P.: N-acetyl-β-glucosaminidase and albuminuria in acromegaly.Horm. Metab. Res., 23, 285 (1991).

    Article  PubMed  Google Scholar 

  12. Stapleton, F. B., Russel, M. D., Chesney, W., Behrmann, A. T., Miller, L. A.: Increased urinary excretion of renal N-acetyl-β-glucosaminidase in hypercalciuria.Amer. J. Dis. Child., 139, 950 (1985).

    CAS  PubMed  Google Scholar 

  13. Tucker, S. M., Pierce, R. J., Price, R. G.: Characterisation of human N-acetyl-β-D-glucosaminidase isoenzymes as an indicator of tissue damage in disease.Clin. Chim. Acta, 102, 29 (1980).

    Article  CAS  PubMed  Google Scholar 

  14. Wehr, H., Czartoryska, B., Górska, D., Matsumoto, H.: Serum β-hexosaminidase and α-mannosidase activities as markers of alcohol abuse.Clin. Exp. Res., 15, 13 (1991).

    Article  CAS  Google Scholar 

  15. Wellwood, J. M., Ellis, B. G., Price, R. G., Hammond, K., Thompson, A. E., Jones, N. F.: Urinary N-acetyl-β-D-glucosaminidase activities in patients with renal disease.Br. Med. J., 3, 408 (1975).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  16. West, B. C., Dunphy, Ch. H., Moore, C. A.: Human neutrophil N-acetyl-β-D-glucosaminidase: Granule localization.J. Lab. Clin. Med., 104, 60 (1984).

    CAS  PubMed  Google Scholar 

  17. Zoch, A., Zwierz, W.: Activity of N-acetyl-beta-D-glucosaminidase (NAG) in blood serum and urine of children with lipoid nephrosis.Pol. Tyg. Lek., 37, 333 (1982).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Toxicology, Medical Academy, Wrocław, Poland

    Z. Marchewka, J. Lembas-Bogaczyk & K. Jacyszin

  2. Department of Nephrology, Medical Academy, Wrocław, Poland

    J. Kuźniar

Authors
  1. Z. Marchewka
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Kuźniar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. Lembas-Bogaczyk
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. K. Jacyszin
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Marchewka, Z., Kuźniar, J., Lembas-Bogaczyk, J. et al. N-acetyl-B-D-glucosaminidase isoenzymes in the diagnosis of poisoning and kidney diseases. Int Urol Nephrol 26, 229–236 (1994). https://doi.org/10.1007/BF02768292

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02768292

Keywords

Search

Navigation