Summary
We studied whether intracoronary Ca administration after β-blockade would increase the internal Ca recirculation fraction (RF) analogously to the Ca administration before β-blockade. This was performed in excised cross-circulated canine hearts. We analyzed the exponential decay component of the postextrasystolic potentiation (PESP) following a spontaneous extrasystole. All the PESPs decayed in alternans with atrial pacing at a constant rate. We obtained the time constant (τe) of the monoexponential decay component of the alternans PESP. An increment of intracoronary Ca by 1.5mmol/1 enhanced the left ventricular contractility indexE max (end-systolic maximum elastance) by 2.5 times before and after β-blockade with propranolol. The intracoronary Ca after β-blockade slightly but significantly increased τe, and hence increased RF calculated from τe by RF=exp(−1/τe). This was analogous to the slightly increased τc and RF with Ca before β-blockade. We speculate that the myocardial cyclic AMP-dependent phosphorylation level would not significantly alter the effect of intracoronarily administered Ca on myocardial Ca handling, in terms of τe and RF.
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This study was partly supported by Grants-in-Aid for Scientific Research (07508003, 09470009, 10770307, 10558136, 10877006) from the Ministry of Education, Science, Sports and Culture, a Research Grant for Cardiovascular Diseases (7C-2) from the Ministry of Health and Welfare, 1997 and 1998 Frontier Research Grants for Cardiovascular System Dynamics from the Science and Technology Agency, and research grants from the Ryobi Teien Foundation, the Mochida Memorial Foundation, and the Nakatani Electronic Measuring Technology Association, all of Japan.
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Hosogi, S., Araki, J., Syuu, Y. et al. Calcium equally increases the internal calcium recirculation fraction before and after β-blockade in canine left ventricles. Heart Vessels 12, 280–286 (1997). https://doi.org/10.1007/BF02766804
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DOI: https://doi.org/10.1007/BF02766804