Abstract
The specific therapeutic effectiveness of a single intravitreal injection of native and immobilized recombinant prourokinase is assessed in lensvitrectomized rabbits with the fibrinoid syndrome. The agents almost completely lyse the fibrin clot within 1–8 h. Heteroprotein conjugates of protein kinase with molecular weight of 900 kD and high fibrinolytic activity at 2–20-fold lower doses of the enzyme are preferable. Native and immobilized prourokinase are recommended for clinical use in case of intraocular fibrin formation after lensvitrectomy and other operations.
Similar content being viewed by others
References
V. F. Danilichev, S. V. Kol’tsova, and E. V. Boiko,Oftal’mol. Zh., No. 5–6, 303–307 (1993).
V. F. Danilichev and I. B. Maksimov,Ocular Injuries and Diseases: Use of Enzymes and Peptide Bioregulators [in Russian], Minsk (1994).
L. V. Zobina and A. D. Romashchenko,Vestn. Oftal’mol.,103, No. 1, 51–57 (1987).
G. P. Kleking and I. N. Bokarev,Klin. Med.,68, No. 6, 13–19 (1990).
S. V. Kol’tsova,Bioorgan. Khimiya,21, No. 6, 408–420 (1995).
S. V. Kol’tsova, E. V. Boiko, N. P. Kuznetsova, and V. F. Danilichev,Vopr. Med. Khimii,41, No. 3, 13–17 (1995).
N. P. Kuznetsova, R. N. Mishaeva, S. V. Kol’tsova,et al., Bioorgan. Khimiya,19, No. 7, 871–879 (1993).
E. V. Chazov, R. A. Gundorova, A. D. Romashchenko,et al.,Vestn. Oftal’mol., No. 4, 61–64 (1982).
G. J. Jaffe, G. A. Abrams, G. A. Williams, and D. P. Han,Ophthalmology,97, 184–189 (1990).
M. J. Poznansky,Methods Enzymol,137, Pt. D., 566–574 (1988).
K. Schmitz, J.-H. Greite, and E. M. Bartenschlager,Germ. J. Ophthalmol.,4, 75–79 (1995).
V. P. Torchilin, A. V. Maksimenko, and A. V. Mazaev,Methods Enzymol.,137, Pt. D., 552–566 (1988).
M. Verstraete and D. Collen,Blood,67, 1529–1541 (1986).
Author information
Authors and Affiliations
Additional information
Translated fromByulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 123, No. 2, pp. 201–204, February, 1997
Rights and permissions
About this article
Cite this article
Boiko, E.V., Danilichev, V.F. & Kol’tsova, S.V. Experimental validation for the use of recombinant prourokinase and its immobilized forms in the treatment of postoperative fibrinoid syndrome in ophthalmology. Bull Exp Biol Med 123, 174–177 (1997). https://doi.org/10.1007/BF02766444
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02766444