Abstract
The activities of superoxide dismutase and guaiacol-dependent peroxidase were studied in the ontogenesis of recessive homozygous mutants ofArabidopsis thaliana Heynh.le-2 andnfz24, which are characterized by two- to threefold increases in tolerance to the herbicide norflurazone. The mutantsle-2 andnfz24 differed from the initial race Dijon in some phenotypic features, duration of ontogenetic stages, and dynamics of the superoxide dismutase and peroxidase activities in ontogenesis. A single treatment of plants with norflurazone induced an accelerated increase in the level of both enzymes in the mutants as compared to the wild type plants. Under the conditions of multiple treatment with norflurazone, the mutantsle-2 andnfz24 displayed a higher tolerance to the bleaching effect of the herbicide and were characterized by a higher level of superoxide dismutase. The data obtained suggest that the superoxide dismutase and peroxidase activities are controlled by both ontogenetic factors and stress signals. Mutations in the linesle-2 andnfz24 increase sensitivity to a stress signal or increase efficiency of an adaptive response due to long-term maintenance of a high level of the antioxidant enzymes under the conditions of stress.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Asada, K., Production and Action of Active Oxygen Species in Photosynthetic Tissues,Causes of Photooxidative Stress and Amelioration of Defense Systems in Plants, Foeyer, C.H. and Mullineaux, P.M., Eds., Boca Raton: CRC, 1994, pp. 77–104.
Bartosz, G., Oxidative Stress in Plants,Acta Physiol. Plant., 1997, vol. 19, no. 1, pp. 47–64.
Bowler, C., Van Montagu, M., and Inzé, D., Superoxide Dismutase and Stress Tolerance,Annu. Rev. Plant Physiol. Plant Mol. Biol., 1992, vol. 43, pp. 83–116.
Casano, L.M., Martin, M., and Sabater, B., Sensitivity to Superoxide Dismutase Transcript Levels and Activities to Oxidative Stress Is Lower in Mature-Senescent than in Young Barley Leaves,Plant Physiol., 1994, vol. 106, pp. 1033–1039.
Chaloupkova, K., and Smart, C.C., The Abscisic Acid Induction of a Novel Peroxidase Is Antagonized by Cytokinin inSpirodela polyrrhiza L.,Plant Physiol., 1994, vol. 105, pp. 497–507.
Donahue, J.L., Ashby, G.A., Thorneley, R.N.F.,et al., Responses of Antioxidants to Paraquat in Pea Leaves,Plant Physiol., 1997, vol. 113, pp. 249–257.
Ezhova, T.A., Soldatova, O.P., Ondar, U.N.,et al., Dwarf Mutants ofArabidopsis thaliana (L.) Heynh. Tolerant to Norflurazone as Objects of Studying Resistance against Oxidative Stress,Fiziol. Rast., 1997a, vol. 44, no. 5, pp. 665–670.
Ezhova, T.A., Ondar, U.N., Soldatova, O.P., and Mamanova, L.B., Genetic and Physiological Studies of Dwarf Mutants ofArabidopsis thaliana (L.) Heynh.Ontogenez, 1997b, vol. 28, no. 5, pp. 344–351.
Gazaryan, I.G. and Lagrimini, L.M., Tobacco Anionic Peroxidase Overexpressed in Transgenic Plants: Aerobic Oxidation of Indole-3-acetic Acid,Phytochemistry, 1996, vol. 42, pp. 1271–1278.
Gazaryan, I.G., Ashby, G.A., Thorneley, R.N.F.,et al., Unusual Kinetic Properties of Anionic Tobacco Peroxidase Related to the Mechanism of Oxidation of Indole-3-acetic Acid,Appl. Biochem. Biotechnol., 1997, vol. 61, pp. 1–12.
Grisebach, H., Lignins,The Biochemistry of Plants, Stumpf, P.K. and Conn, E.E., Eds., New York: Academic, 1981, pp. 457–77.
Jespersen, H.M., Kjaersgard, I.V., Ostergaard, L.,et al., From Sequence Analysis of Three Novel Ascorbate Peroxidases fromArabidopsis thaliana to Structure, Function and Evolution of Seven Types of Ascorbate Peroxidase,Biochem. J., 1997, vol. 326, no. 2, pp. 305–310.
Kardish, N., Magal, N., and Aviv, D., The Tomato Gene for the Chloroplastic Cu, Zn Superoxide Dismutase Regulation of Expression Imposed in Transgenic Tobacco Plants by a Short Promoter,Plant Mol. Biol., 1994, vol. 25, pp. 887–897.
Kliebenstein, D.J., Monde, R.A., and Last, R.L., Superoxide Dismutase inArabidopsis: An Eclectic Enzyme Family with Disparate Regulation and Protein Localization,Plant Physiol., 1998, vol. 18, no. 2, pp. 637–650.
Kvaratshelia, M., Winkel, C., and Thorneley, N., Purification and Characterisation of a Novel Class III Peroxidase Isoenzyme from Tea Leaves,Plant Physiol., 1997, vol. 114, pp. 1237–1245.
Lurie, S., Fallic, E., Handros, A.,et al., The Possible Involvement of Peroxidase in Resistance toBotrytis cinerea in Heat Treated Tomato Fruit,Physiol. Mol. Plant. Pathol., 1997, vol. 50, pp. 141–149.
McClung, C.R., Regulation of Catalases inArabidopsis, Free Radic. Biol. Med., 1997, vol. 23, no. 3, pp. 489–496.
Perl-Treves, R. and Galun, E., The Tomato Cu, Zn Superoxide Dismutase Genes Are Developmentally Regulated and Respond to Light and Stress,Plant Mol. Biol., 1991, vol. 17, pp. 745–760.
Planz, H., Oxidation of IAA by Extracellular Peroxidase,3rd Int. Symp. “Plant Peroxidase: Biochemistry and Physiology”, 1993, pp. 169–174.
Rao, M.V., Poliyath, G., and Ormrod, D.P., Ultraviolet-B- and Ozone-Induced Biochemical Changes in Antioxidant Enzymes ofArabidopsis thaliana, Plant Physiol., 1996, vol. 110, pp. 125–136.
Soldatova, O.P., Ezhova, T.A., Ondar, U.N.,et al., Arabidopsis thaliana (L.) Heynh. Mutants Tolerant to the Inhibitor of Carotenoid Biosynthesis Norflurazone,Genetika, 1996, vol. 32, no. 7, pp. 956–961.
Tanaka, K., Gene Structures and Expression Control of Active Oxygen Scavenging Enzymes in Mice,Stress Responses of Photosynthetic Organisms, Satoh, K. and Murata, N., Eds., Amsterdam: Elsevier, 1998, p. 168.
Ulmasov, T., Ohmiya, A., Hagen, G.,et al., The Soybean CH2/4 Gene that Encodes a Glutathione S-Transferase Has a Promoter that Is Activated by a Wide Range of Chemical Agents,Plant Physiol., 1995, vol. 108, pp. 919–927.
Williamson, J. and Scandalios, J.G., Differential Response of Maize Catalases to Abscisic Acid: vp1 Transcriptional Activator Is Not Required for Abscisic Acid-Regulated Cat1 Expression,Proc. Natl. Acad. Sci. USA, 1992, vol. 89, pp. 8842–8846.
Yamasaki, H., Sakihama, Y., and Ikehana, N., Flavonoid-Peroxidase Reaction as Detoxification Mechanism of Plant Cells against H2O2.Plant Physiol., 1997, vol. 115, pp. 1405–1412.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ezhova, T.A., Soldatova, O.P., Mamanova, L.B. et al. A study of the activity of enzymes of the antioxidant system in ontogenesis ofArabidopsis thaliana mutants tolerant to norflurazone. Russ J Dev Biol 31, 32–37 (2000). https://doi.org/10.1007/BF02758753
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02758753