Abstract
We describe a method for the detection of specific antibody-producing cells from either in vitro or in vivo immunization. These techniques are especially useful for detecting antibodies from developing hybridomas. We have successfully used the system to detect isotype-specific antibodies to a variety of bacterial antigens which were produced by heterohybridomas.
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References
Czerkinsky, C., Nilsson, L. A., Tarkowski, A., Koopman, W. J., Mestecky, J., and Ouchterlony, O. (1988) The enzyme-linked immunospot assay for detection of specific antibody-secreting cells: methodlogy and applicability, inTheoretical and Technical Aspects of ELISA and Other Solid Phase Immunoassays (Kemeny, D. M. and Challacombe, S. J., eds.), Wiley, Chichester, UK, pp. 217–239.
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Cooper, M.D., Kirkpatrick, R. Measurement of immunoglobulin synthesis using the ELISPOT assay. Mol Biotechnol 8, 169–171 (1997). https://doi.org/10.1007/BF02752261
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DOI: https://doi.org/10.1007/BF02752261