Summary
The present study describes the effect of nutrient secretagogues upon insulin release from an insulinoma derived B-cell line RINm5F. Glucose failed to stimulate insulin release, it did however increase the rate of glucose oxidation by some 16 fold. Glyceraldehyde stimulated insulin release in a concentration dependent manner, maximum stimulation occurring at 20 mmol/l. The response to glyceraldehyde was suppressed by calcium channel blockade and calmodulin inhibition. Insulin release was also stimulated by the amino acids leucine and arginine, maximum stimulation for both amino acids occurring at 15 mmol/l. The present study confirms the usefulness of this cell line as a model for the study of insulin secretion.
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References
Chesney, T. Mc. C., Schofield, J. G.: Studies on the secretion of pancreatic glucagon — Diabetes18, 627, 1969.
Chick W. L., Warren S., Chute R. N., Like A. A., Lauris V., Kitchen K. C.: A trasplantable insulinoma in the rat — Proc. nat. Acad. Sci. (Wash.)74, 628, 1977.
Devis G., Somers G., Van-Obberghen E., Malaisse W. J.: Calcium antagonists and islet function: inhibition of insulin secretion by verapamil — Diabetes24, 547, 1975.
Fleckenstein A.: Specific pharmacology of calcium in myocardium, cardiac pacemakers and vascular smooth muscle — Ann. Rev. Pharmacol. Toxicol.17, 149, 1977.
Gazdar, A. F., Chick, W. L., Oie K. H., Sims H. L., King D. L., Weir G. C., Lauris V.: Continuous, clonal, insulin and somatostatin secreting cell lines established from a transplantable rat islet cell tumor — Proc. nat. Acad. Sci. (Wash.)77, 3519, 1980.
Grodsky, C. M.: Insulin and the pancreas — Vitam. and Horm.28, 37, 1970.
Groix M.-H., Sener, A., Malaisse-Lagae F., Malaisse W. J.: Glucose metabolism in insulin producing tumoral cells — Arch. Biochem. Biophys.241, 561,1985.
Halban P. A., Praz G. A., Wollheim C. B.: Abnormal glucose metabolism accompanies failure of glucose to stimulate insulin release from a rat pancreatic cell line (RINm5F). —Biochem. J.212, 439, 1983.
Hales C. N., Randle, P. J.: Immunoassay of insulin with insulin antibody precipitate — Biochem. J.88, 137, 1963.
Hunter W. M., Greenwood F. C.: Preparation of 131-labelled human growth hormone of high specific activity — Nature (Lond.)194, 495, 1962.
Hussain M., Atkins T. W.: The effect of digitoxose on insulin release, glucose oxidation and oxygen consumption by islets of lean and genetically obese diabetic (ob/ob) mice — Biochem. biophys. Res. Commun.129, 358, 1985.
Korman L. Y., Bhathena S. J., Voyles N. R., Oie H. K., Recant L.: Characteristics of the interaction of the glucagon receptor, cAMP, and insulin secretion in parent cells and clone 5F of a cultured rat insulinoma — Diabetes34, 717, 1985.
Lambert D. G., Hughes K., Atkins T. W.: Insulin release from a cloned hamster B-cell line (HIT-T15). The effects of glucose, amino acids, sulphonylureas and colchicine — Biochem. biophys. Res. Commun.140, 616, 1986.
Levin R. M., Weiss B.: Binding of trifluoperazine to the calcium-dependent activator of cyclic nucleotide phosphodiesterase — Molec. Pharmacol.13, 690, 1977.
Niki A., Niki H.: Effects of anomers of D-glucose on insulin release from perfused rat pancreas — Biochem. Res.1, 189, 1980.
Praz G. A., Halban P. A., Wollheim C. B., Blondel B., Strauss A. J., Renold A. E.: Regulation of immunoreactive insulin release from a rat cell line (RINm5F) — Biochem. J.210, 345, 1983.
Santerre R. F., Cook R. A., Crisel R. M. D., Sharp J. D., Schmidt R. J., Williams D. C., Wilson C. P.: Insulin synthesis in a clonal cell line of simian virus 40-transformed hamster pancreatic B-cells — Proc. nat. Acad. Sci. (Wash.)78, 4339, 1981.
Sener A., Malaisse W. J.: Nutrient metabolism in islet cells — Experientia (Basel)40, 1026, 1984.
Uchida S., Watanabe S., Aizawa T., Furuno A., Muta T.: Polyoncogenecity in insulinoma-inducing ability of BK virus, a human papovirus in Syrian golden hamsters — J. nat. Cancer Inst.63, 119, 1979.
Wollheim C. B., Ullrich S., Pozzan T.: Glyceraldehyde but not cyclic AMP-stimulated insulin release is preceded by a rise in cytosolic free calcium — FEBS Letters177, 17, 1984.
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Lambert, D.G., Atkins, T.W. Nutrient induced insulin release from an insulinoma derived B-cell line. Acta diabet. lat 24, 299–304 (1987). https://doi.org/10.1007/BF02742961
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DOI: https://doi.org/10.1007/BF02742961