Summary
The interhormonal relationship within the pancreatic islets have been studied by previous investigators, but the cellular interplay and the sequence of events in the islet cell's response to stimulators has remained unclear. In the present study, pancreatic islets were isolated by collagenase digestion from normal and streptozotocin-diabetic hamsters the latter being maintained with insulin treatment. The diabetic animals were used to provide A- and B-cell enriched islets. The islets from normal and diabetic hamsters were cultured in medium 199 plus 10% fetal calf serum with 0.8 or 5 mg/ml glucose. The cultures were maintained for up to seven days
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Shieh, JC., Dunbar, J.C. Insulin, glucagon and somatostatin secretion by cultured islets from normal and diabetic hamsters. Acta diabet. lat 24, 287–297 (1987). https://doi.org/10.1007/BF02742960
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DOI: https://doi.org/10.1007/BF02742960