Skip to main content
SpringerLink
Log in

Retrospective glycemic status of diabetic patients: Glycosylation of blood proteins in diabetes and chronic renal failure

  • Original Contributions
  • Published:
Acta diabetologia latina Aims and scope Submit manuscript

Summary

In vivo andin vitro studies were carried out to evaluate the clinical application of glycosylated hemoglobin and plasma proteins in the diagnosis and management of diabetes mellitus. Glycosylated hemoglobin registered an almost 80% fall in diabetic patients following controlled glycemia for two months while glycosylated, plasma protein level registered an 80% fall in the patients after fifteen days of blood glucose homeostasis. Human serum proteins were glycosylatedin vitro and glycosylation was linearly proportional to the glucose concentration and incubation time. Polyacrylamide gel electrophoresis of glycosylated serum proteins revealed that albumin and transferrin are the major, proteins that are significantly glycosylated. Glycosylated hemoglobin and plasma protein levels were also increased in chronic renal failure patients without any history of diabetes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bolli G., Compagnucci P., Cartechini M. G., Santeusanio F., Cirotto C., Scionti L., Brunetti P.: HbA1 in the subjects with abnormal glucose tolerance but normal fasting plasma glucose—Diabetes29, 272, 1980.

    Article  PubMed  CAS  Google Scholar 

  2. Brownlee M., Cerami A.: The biochemistry of the complications of diabetes mellitus—Ann. Rev. Biochem.50, 385, 1981.

    Article  PubMed  CAS  Google Scholar 

  3. Brownlee M., Vlassara H., Cerami A.: Nonenzymatic glycosylation and the pathogenesis of diabetic complications—Ann. intern. Med.101, 527, 1984.

    PubMed  CAS  Google Scholar 

  4. Bunn H. F., Gabbay K. H., Gallop P. M.: The glycosylation of hemoglobin: relevance to diabetes mellitus—Science200, 21, 1978.

    Article  PubMed  CAS  Google Scholar 

  5. Casparie A. F., Miedema K.: Glycosylated haemoglobin in diabetes and renal failure—Lancetii, 758, 1977.

    Article  Google Scholar 

  6. Cudworth A. G., Bodansky H. J., West K. M.: Genetic and metabolic factors in relationship to the prevalence and severity of diabetic complications. In:Keen H., Jarrett J. (Eds): Complications of diabetes. 2nd edition. Edward Arnold, London, 1982; p. 1.

    Google Scholar 

  7. Day J. F., Thornbur R. W., Thorpe S. R., Baynes J. W.: Nonenzymatic glucosylation of rat albumin: Studiesin vitro andin vivo.—J. biol. Chem.254, 9394, 1979.

    PubMed  CAS  Google Scholar 

  8. Day J. F., Thorpe S. R., Baynes J. W.: Nonenzymatic glucosylation of rat serum proteinsin vitro andin vivo.—Fed. Proc..38, 418, 1979.

    Google Scholar 

  9. de Boer M. J., Miedema K., Casparie A. F.: Glycosylated hemoglobin in renal failure.— Diabetologia18, 437, 1980.

    Article  PubMed  Google Scholar 

  10. Dolhofer R., Wieland O. H.: Increased glycosylation of serum albumin in diabetes mellitus.—Diabetes29, 417, 1980.

    Article  PubMed  CAS  Google Scholar 

  11. Elder E., Kennedy L.: Rapid accurate colorimetric assay of non-enzymatically glycosylated serum proteins.—Diabetologia.24, 70, 1984.

    Google Scholar 

  12. Gabbay K. H.: Hyperglycemia, polyol metabolism and complications of diabetes mellitus.—Ann. Rev. Med.26, 521, 1975.

    Article  PubMed  CAS  Google Scholar 

  13. Gabbay K. H., Hasty K., Breslow J. L., Ellison R. C., Bunn H. F., Gallop P. M.: Glycosylated hemoglobins and long term blood glucose control in diabetes mellitus.—J. clin. Endocrinol.44, 859, 1977.

    Article  CAS  Google Scholar 

  14. Gabbay K. H., Sosenko J. M., Banuchi G. H., Mininsohn M. J., Fluckiger R.: Glycosylated hemoglobins: increased glycosylation of hemoglobin A in diabetic patients.—Diabetes.28, 337, 1979.

    Article  PubMed  CAS  Google Scholar 

  15. Goldstein D. E., Peth S. B., England J. D., Hess R. L., Da Costa J.: Effects of acute changes in blood glucose on HbA1c—Diabetes.29, 623, 1980.

    Article  PubMed  CAS  Google Scholar 

  16. Gragnoli G., Tanganelli I., Signorini A. M., Tarli P., Paoli C.: Non-enzymatic glycosylation of serum proteins as an indicator of diabetic control.—Diabetologia.22, 224, 1982.

    PubMed  CAS  Google Scholar 

  17. Guthrow C. E., Morris M. A., Day J. F., Thorpe S. R., Baynes J. W.: Enhanced nonenzymatic glucosylation of human serum albumin in diabetes mellitus.—Proc. nat. Acad. Sci. (Wash.)76, 4258, 1979.

    Article  CAS  Google Scholar 

  18. Howard C.F. Jr: Correlations of hemoglobin A1c and metabolic status in non-diabetic, borderline diabetic, and diabeticMacaca nigra.—Diabetes31, 1105, 1982.

    Article  PubMed  CAS  Google Scholar 

  19. Kemp S. F., Creech R. H., Horn T. R.: Glycosylated albumin and transferrin: short term markers of blood glucose control.—J. Pediatr..105, 394, 1984.

    Article  PubMed  CAS  Google Scholar 

  20. Koenig R. J., Peterson C. M., Jones R. L., Saudek C., Lehrman M., Cerami A.: Correlation of glucose regulation and hemoglobin A1c in diabetes mellitus.—New Engl. J. Med.295, 417, 1976.

    Article  PubMed  CAS  Google Scholar 

  21. Koenig R. J., Peterson C. M., Kilo C., Cerami A., Williamson J. R.: Haemoglobin A1c as an indicator of the degree of glucose intolerance.—Diabetes25, 230, 1976.

    Article  PubMed  CAS  Google Scholar 

  22. Lowry D. H., Rosebrough N. J., Farr A. L., Randell R. J.: Protein measurement with the folin phenol reagent.—J. biol. Chem..193, 265, 1951.

    PubMed  CAS  Google Scholar 

  23. Marble A., Camerini-Davalos R.: Clinical experience with sulfonylurea compounds in diabetes.—Ann. N.Y. Acad. Sci..71, 239, 1957.

    Article  PubMed  CAS  Google Scholar 

  24. Mc Farland K. F., Catalano E. W., Day J. F., Thorpe S. R., Bayne J. W.: Nonenzymatic glucosylation of serum proteins in diabetes mellitus.—Diabetes.28, 1011, 1979.

    Article  CAS  Google Scholar 

  25. McVerry B. A., Fisher C., Hopp A., Huchens E. R.: Production of pseudo-diabetic renal glomerular changes in mice after repeated injections of glucosylated proteins.—Lanceti, 738, 1980.

    Article  Google Scholar 

  26. Morris A. M., Grandis S. A., Jean Litton B. S.: The correlation of glycosylated serum proteins and glycosylated hemoglobin concentrations with blood glucose in diabetic pregnancy.—Amer J. Obstetr. Gynecol.153, 257, 1985.

    CAS  Google Scholar 

  27. National Diabetes Data Group: Classification and diagnosis of diabetes mellitus and other categories of glucose intolerance.—Diabetes.28, 1039, 1979.

    Google Scholar 

  28. Oimoni M., Ishikawa K., Kawasaki T., Kubota S., Yoshimura Y., Baba S.: Glycosylated haemoglobin in renal failure.—Diabetologia21, 163, 1981.

    Google Scholar 

  29. Pecoraro R. E., Graf R. J., Halter J. B., Beiter H., Porte D. Jr.: Comparison of a colorimetric assay for glycosylated hemoglobin with ion-exchange chromatography.—Diabetes28, 1120, 1979.

    Article  PubMed  CAS  Google Scholar 

  30. Raabo E., Terkildsen T. C.: On the enzymatic determination of blood glucose.—Scand. J. clin. Lab. Invest..12, 402, 1960.

    Article  PubMed  CAS  Google Scholar 

  31. Rahbar S., Blumenfeld O., Ranney H. M. Studies of an unusual hemoglobin in patients with diabetes mellitus.—Biochem. biophys. Res. Commun..36, 838, 1969.

    Article  PubMed  CAS  Google Scholar 

  32. Schernthaner G., Stummvoll K. H., Muller M. M.: Glycosylated hemoglobin in chronic renal failure—Lanceti, 774, 1979.

    Article  Google Scholar 

  33. Sigma Technical Bulletin: Total hemoglobin in whole blood. No. 525, p.1, 1977.

  34. Trivelli L. A., Ranney H. M., Lai H.-T.: Hemoglobin components in patients with diabetes mellitus—New Engl. J. Med.284, 353, 1971.

    Article  PubMed  CAS  Google Scholar 

  35. Urbanowski J. C., Cohenford M. A., Dain J. A.: Nonenzymatic galactosylation of human serum albumin.—J. biol. Chem.257, 111, 1982.

    PubMed  CAS  Google Scholar 

  36. West K. M.: Hyperglycemia as a cause of long term complications In:Keen H., Jarrett J. (Eds): Complications of diabetes. 2nd edition. Edward Arnold, London, 1982; p. 13.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Biochemistry, Central Drug Research Institute, 226 001, Lucknow, U.P., India

    Kshama Kumari, V. Bansal, Anil K. Rastogi & Maharaj K. Sahib

  2. Department of Medicine, King George's Medical College, Lucknow, India

    Jagmohan & C. G. Agarwal

Authors
  1. Kshama Kumari
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V. Bansal
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jagmohan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. C. G. Agarwal
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Anil K. Rastogi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Maharaj K. Sahib
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

C.D.R.I. Communication no 3908.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kumari, K., Bansal, V., Jagmohan et al. Retrospective glycemic status of diabetic patients: Glycosylation of blood proteins in diabetes and chronic renal failure. Acta diabet. lat 24, 91–99 (1987). https://doi.org/10.1007/BF02742847

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02742847

Key-words

Search

Navigation