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The role of insulin-like growth factor-I (IGF-I) and estradiol in rabbit corpus luteum progesterone production

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Abstract

To determine whether insulin-like growth factor-I (IGF-I) plays a role in rabbit corpus luteum (CL) physiology the authors examined: IGF-I expression, the effect of IGF-I on progesterone (P) production in vitro, and the interaction of IGF-I with estradiol, the primary luteotropin in the rabbit. Northern blot analysis revealed that IGF-I mRNA is present in the rabbit CL throughout pseudopregnancy. An intact ovarian in vitro perfusion model and dispersed luteal cell culture were used to determine the effects of IGF-I on P production and interactions with estradiol. IGF-I significantly stimulated P production compared to control medium by the isolated, intact perfused rabbit ovary and by dispersed, cultured luteal cells. Estradiol alone did not stimulate P production in vitro. Estradiol did augment IGF-I-stimulated P production in the intact perfused ovary and in luteal cell culture. These findings support a role for IGF-I in rabbit CL P production.

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Authors and Affiliations

  1. Department of Gynecology and Obstetrics, School of Medicine, Johns Hopkins University, Baltimore, MD

    Serena H. Chen, Vanna Zanagnolo, Sangchai Preutthipan, Sandra B. Goodman & Arunasalem M. Dharmarajan

  2. Department of Population Dynamics, School of Hygiene and Public Health, Johns Hopkins University, Baltimore, MD

    Kenneth P. Roberts

Authors
  1. Serena H. Chen
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  2. Vanna Zanagnolo
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  3. Sangchai Preutthipan
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  4. Kenneth P. Roberts
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  5. Sandra B. Goodman
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  6. Arunasalem M. Dharmarajan
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Chen, S.H., Zanagnolo, V., Preutthipan, S. et al. The role of insulin-like growth factor-I (IGF-I) and estradiol in rabbit corpus luteum progesterone production. Endocr 6, 73–77 (1997). https://doi.org/10.1007/BF02738805

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  • DOI: https://doi.org/10.1007/BF02738805

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