Skip to main content
Log in

New cyclomaltodextrin glucan transferases produced by bacillus macerans

  • Published:
Applied Biochemistry and Microbiology Aims and scope Submit manuscript

Abstract

—For the first time, microorganisms producing cyclomaltodextrin glucantransferaseglucan transferases (CGT, EC 2.4.1.19) were isolated from soil samples of various ecogeographical regions. These microorganisms were identified asBacillus macerans. The enzymes were purified by affinity chromatography on an α-cyclodextrin polymer and gel filtration on Biogel P-150 and proved to be electrophoretically homogeneous. Some of their physicochemical and biochemical properties are reported.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Szejtli, J.,Cyclodextrins and Their Inclusion Complexes, Budapest: Akad. Kiado, 1982.

    Google Scholar 

  2. Duchen, D.,New Trends in Cyclodextrins and Derivatives, Paris: Editions de Sante, 1991.

    Google Scholar 

  3. Abelyan, V.A., Adamyan, M.O., Abelyan, L.A., Bala-yan, A.M., and Afrikyan, E.G.,Biokhimiya (Moscow), 1995, vol. 60, no. 6, pp. 891–897.

    Google Scholar 

  4. Abelyan, V.A., Adamyan, M.A., and Abelyan, L.A.,Biokhimiya (Moscow), 1995, vol. 60, no. 6, pp. 898–904.

    Google Scholar 

  5. Abelyan, V.A., Afyan, K.B., Avakyan, Z.G., Mel-kumyan, A.G., and Afrikyan, E.G.,Biokhimiya (Moscow), 1995, vol. 60, no. 10, pp. 1600–1680.

    Google Scholar 

  6. Abelyan, V.A., Avakyan, Z.G., Melkumyan, A.G., Ba-layan, A.M., Uzunyan, L.V., and Gasparyan, A.V.,Biokhimiya (Moscow), 1995, vol. 57, no. 3, pp. 430–437.

    Google Scholar 

  7. Kitahata, S. and Okada, S.,J. Jpn. Soc. Starch Sci., 1982, vol. 29, no. 1, pp. 13–18.

    CAS  Google Scholar 

  8. Binder, F., Huber, O., and Boeck, A.,Gene, 1986, vol. 47, no. 2, pp. 269–277.

    Article  PubMed  CAS  Google Scholar 

  9. Kaneko, T., Hamamoto, T., and Horokoshi, K.,J. Gen. Microbiol., 1988, vol. 134, no. 2, pp. 97–105.

    PubMed  CAS  Google Scholar 

  10. Abelyan, V.A., Dikhtyarev, S.I., and Afrikyan, E.G.,Biokhimiya (Moscow), 1991, vol. 56, no. 9, pp. 1583–1590.

    CAS  Google Scholar 

  11. Park, C.S., Park, K.H., and Kim, S.N.,Agric. Biol. Chem., 1989, vol. 53, no. 10, pp. 1167–1169.

    CAS  Google Scholar 

  12. Davis, B.I.,Ann. N.Y. Acad. Sci., 1964, vol. 121, pp. 404–427.

    Article  PubMed  CAS  Google Scholar 

  13. Laemmly, V.K.,Nature (London), 1970, vol. 227, no. 7, pp. 680–685.

    Article  Google Scholar 

  14. Makela, M.J. and Korpela, T.K.,Biochem. Biophys. Methods, 1988, vol. 15, no. 5, pp. 307–318.

    Article  CAS  Google Scholar 

  15. Abelyan, V.A., Gasparyan, A.V., Avakyan, Z.G., and Afrikyan, E.G.,Biokhimiya (Moscow), 1991, vol. 56, no. 9, pp. 1578–1582.

    CAS  Google Scholar 

  16. Lejeune, A., Sakaguchi, K., and Imanaka, T.,Anal. Biochem., 1989, vol. 181, no. 1, pp. 6–11.

    Article  PubMed  CAS  Google Scholar 

  17. Vikmon, M.,Proc. 1st Int. Symp. on Cyclodextrins, Sze-jtli, J., Ed., Budapest: D. Reidel, 1982, pp. 69–74.

    Google Scholar 

  18. Abelyan, V.A., Yamamoto, T., and Afrikyan, E.G.,Biokhimiya (Moscow), 1994, vol. 59, no. 6, pp. 778–787.

    Google Scholar 

  19. Turkova, J.,Affinity Chromatography, Amsterdam: Elsevier, 1978. Translated under the titleAffinnaya khromatografiya, Moscow: Mir, 1980.

    Google Scholar 

  20. Kimura, K., Kataoka, S., Ishii, Y., Takano, T., and Koba-yashi, S.,Biochem. Biophys. Res. Commun., 1989, vol. 161, no. 10, pp. 1273–1297.

    Article  PubMed  CAS  Google Scholar 

  21. Kitahata, S. and Okada, S.,Agric. Biol. Chem., 1974, vol. 38, no. 2, pp. 390–393.

    Google Scholar 

  22. Kobayashi, S., Kainuma, K., and Suzuki, S.,Carbohydr. Res., 1978, vol. 61, no. 2, pp. 229–238.

    Article  PubMed  CAS  Google Scholar 

  23. Depinto, J. and Campbell, L.L.,Biochemistry, 1969, vol. 7, no. 1, pp. 121–125.

    Article  Google Scholar 

  24. Yagi, Y., Sato, M., and Ishikura, T.,J. Jpn. Soc. Starch Sci., 1986, vol. 33, no. 3, pp. 144–151.

    CAS  Google Scholar 

  25. Stavn, A. and Granum, P.E.,Carbohydr. Res., 1979, vol. 75, no. 4, pp. 243–250.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Abelyan, V.A., Afyan, K.B. & Manukyan, L.S. New cyclomaltodextrin glucan transferases produced by bacillus macerans. Appl Biochem Microbiol 36, 338–343 (2000). https://doi.org/10.1007/BF02738040

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02738040

Keywords

Navigation