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Change of hemoagglutinin and hemolysin titers in hemolymph of gastropod molluscs in response to immunization with sheep erythrocytes

  • Comparative and Ontogenic Biochemistry
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Abstract

This work deals with analysis of changes of the levels of hemoagglutinins (HA) and hemolysins (HL) in hemolymph of three gastropod species,Planorbius corneus, Lymnea stagna-lis, andAchatina fulica, in response to immunization with sheep erythrocytes (ShE). The levels of HA and HL were determined before immunization and 3, 5, 10, and 15 days after the antigen administration. The HA titer was estimated from hemoagglutination reaction, the HL titer was measured spectrophotometrically from release of hemoglobin from ShE. It has been found that after immunization the titer of HA falls by 72–88% for the first 3–5 days, but returns to the initial values or even exceeds them statistically significantly by the10–15th days. The time of the decrease of the HA titers correlates inversely with the body mass of the animals, while the amplitude of this decrease is directly proportional to the body mass. The HL titer either changes parallel to the HA titer (inL. stagnalis) or increases statistically significantly by 60–65% during the entire period of observation (in two other species studied). It is suggested that inP. coneus andA. fulica, HA and HL are different compounds.

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Authors and Affiliations

  1. Faculty of Biology and Soil Sciences, State University, St. Petersburg

    A. V. Baskakov & A. D. Harazova

  2. Research Institute of Experimental Medicine, Russian Academy of Medical Sciences, St. Petersburg, Russia

    A. V. Polevshchikov & E. G. Krasnodembskii

Authors
  1. A. V. Baskakov
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  2. A. V. Polevshchikov
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  3. A. D. Harazova
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  4. E. G. Krasnodembskii
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Baskakov, A.V., Polevshchikov, A.V., Harazova, A.D. et al. Change of hemoagglutinin and hemolysin titers in hemolymph of gastropod molluscs in response to immunization with sheep erythrocytes. J Evol Biochem Phys 36, 277–281 (2000). https://doi.org/10.1007/BF02737043

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  • DOI: https://doi.org/10.1007/BF02737043

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