Abstract
Two filamentous, nitrogen fixing cyanobacteria were examined for their salt tolerance and sodium (Na+) transport.Anabaena torulosa, a saline form, grew efficiently and fixed nitrogen even at 150 mM salt (NaCl) concentration while,Anabaena L-31, a fresh water cyanobacterium, failed to grow beyond 35 mM NaCl.Anabaena torulosa showed a rapidly saturating kinetics of Na+ transport with a high affinity for Na+ (K m, 0.3 mM).Anabaena L-31 had a much lower affinity for Na+ (Km, 2.8 mM) thanAnabaena torulosa and the pattern of uptake was somewhat different. BothAnabaena spp. exhibited an active Na+ extrusion which seems to be mediated by a Na+-K+ ATPase and aided by oxidative phosphorylation.Anabaena L-31 was found to retain much more intracellular Na+ thanAnabaena torulosa. The results suggest that the saline form tolerates high Na+ concentrations by curtailing its influx and also by an efficient Na+ extrusion, although these alone may not entirely account for its success in saline environment.
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Abbreviations
- DCMU:
-
3-(3, 4-Dichlorophenyl) -1, 1-dimethylurea
- CCP:
-
carbonyl cyanide,m- chlorophenyl hydrazone
- DNP, 2, 4-dinitrophenol:
-
DCCD, N, N′ -dicyclohexylcarbodiimide
- BBOT:
-
2, 5-(5-ditetrabutyl-2-benzoxazolyl)-thiophene
- ATPase:
-
Adenosine triphosphatase
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Apte, S.K., Thomas, J. Sodium transport in filamentous nitrogen fixing cyanobacteria. J Biosci 5, 225–233 (1983). https://doi.org/10.1007/BF02716605
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DOI: https://doi.org/10.1007/BF02716605