Abstract
The plant growth hormones — BA, GA3 or IAA (alone or in combination) in single dose were injected into the centre of intact imbibed seed, germinated in the presence of water and monitored for root length, shoot length, and activities of α-amylase and proteases during a 10-day germination period of Indian bean seeds. A significant increase was noticed in the root length and shoot length compared to the respective controls. Injection of BA, GA3 or IAA alone resulted in a significant increase in the activity of α-amylase. The increase in the activities of proteases (acidic, neutral and alkaline) was not affected with either BA or GA3 during the early stage of germination, but the fall in their activities in the later stages of germination was suppressed. However, in vivo administration of IAA alone or in combination with BA or GA3 resulted in faster and higher development of α-amylase and protease(s) activities. These results indicated that more than one hormone is necessary for inducing the development of α-amylase and proteases at early stages, and also responsible for maintaining the higher activities in the later stages of germination.
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Abbreviations
- BA:
-
Benzyl adenine
- ABA:
-
Abscisic acid
- DMR:
-
Duncan Multiple Range
- GA3 :
-
Gibberellic acid
- IAA:
-
Indole acetic acid
References
Beevers L. 1968. Protein degradation and proteolytic activity in the cotyledons of germinating pea seeds. Phytochemistry. 7: 1837–1844.
Bernfeld P. 1955. Amylases. α- and β-. Method Enzymol. 8: 525–532.
Bewley J.D., Black M. 1994. Seeds: Physiology of development and germination. 2 Ed., New York, London, Plenum Press.
Callis J. 1995. Regulation of protein degradation. The Plant Cell. 7: 845–857.
Cejudo F.C., Murphy G., Chinoy C., Baulcombe C. 1992. A gibberellin-regulated gene from wheat with sequence homology to cathepsin B of mammalian cells. Plant J. 2: 937–978.
Davies H.V., Slack P.T. 1981. The control of food mobilization in the seeds of dicotyledonous plants. New Phytol. 88: 41–51.
Finkelstein R.R., Lynch T.J. 2000. Abscisic acid inhibition of radicle emergence but not seedling growth is suppressed by sugars. Plant Physiol. 122: 1179–1186.
Liu Z., Wang W., Yen Y. 1998. Effect of hormone treatment on root formation and endogenous indole 3-acetic acid and polyamine levels of Glycine max cultivated in vitro. Bot. Bull. Acad. Sin. 39: 113–118.
Marttila S., Porali I., Ho T.H.D., Mikkonen A. 1993. Expression of the 30KD cysteine endoprotease B in germinating barley seeds. Cell Biol. Int. 17: 205–212.
Munoz J.L., Martin L., Nicolas G., Villalobos N. 1990. Influence of endogenous cytokinins on reserve mobilization in cotyledons of Cicer arietinum L. Plant Physiol. 93: 1011–1016.
Muntz K., Belozersky M.A., Dunaevsky Y.E., Schlereth A., Tiedemann J. 2001. Stored proteinases and the initiation of storage protein mobilization in seeds during germination and seedling growth. J Exp Bot. 52: 1741–1752.
Nandi S.K., Palni L.M.S., de Klerk J.M. 1995. The influence of the embryonic axis and cytokinins on reserve mobilization in germinating lupine seeds. J. Exp. Bot. 46: 329–226.
Nandi S.K., Palni L.M.S. 1989. Transport and metabolism of dihydrozeatin riboside in germinating lupine seeds. J. Exp. Bot. 40: 615–621.
Raghuramulu N., Madhavan Nair K., Kalyanasundaram S. 2003. A manual of laboratory techniques, National Institute of Nutrition, Hyderabad, India.
Ramakrishna V., Ramakrishna Rao P. 2005a. Role of embryonic axis in the mobilization of starch during germination of Indian bean (Dolichos lablab L. var. lignosus) seeds. Seed Science and Technology (accepted).
Ramakrishna V., Ramakrishna Rao P. 2005b. Axial control of protein reserve mobilization during germination of Indian bean (Dolichos lablab L.) seeds. Acta Biologica Szegediensis 49(3–4): 23–27.
Swain R.R., Dekker E.E. 1966. Seed Germination studies. II. Pathways for starch degradation in germinating pea seedlings. Biochem. Biophys. Acta. 122: 87–100.
Taneyama M., Okamoto T., Yamane H., Minamikawa T. 2001. Involvement of gibberellins in expression of a cysteine proteinase (SH-EP) in cotyledons of Vigna mungo seedlings. Plant Cell Physiol. 42: 1290–1293.
Taneyama M., Okamoto T., Yamauchi D., Minamikawa T. 1996. Development of endopeptidase activity in cotyledons of Vigna mungo seedling: Effects of exogenously applied end products and plant hormones. Plant Cell Physiol. 37: 19–26.
Tiedemann J., Neubohn B., Muntz K. 2000. Different functions of vicillin and legumin are reflected in the histopattern of globulin mobilization during germination of vetch (Vicia sativa L.). Planta 211: 1–12.
Tiedemann J., Schlereth A., Muntz K. 2001. Differential tissue-specific expression of cystein proteinases forms the basis for the fine-tuned mobilization of storage globulin during and after germination in legume seeds. Planta 212: 728–38.
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Ramakrishna, V., Rao, P.R. Effect of in vivo administered plant growth hormones on the development of amylase and protease during germination of indian bean (Dolichos lablab L. var. lignosus) seeds. Acta Physiol Plant 28, 245–250 (2006). https://doi.org/10.1007/BF02706537
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DOI: https://doi.org/10.1007/BF02706537