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cDNA cloning and characterization of a mannose-binding lectin fromZingiber officinaleRoscoe (ginger) rhizomes

Abstract

Using RNA extracted fromZingiber officinale rhizomes and primers designed according to the conservative regions of monocot mannose-binding lectins, the full-length cDNA ofZ. officinale agglutinin (ZOA) was cloned by rapid amplification of cDNA ends (RACE). The full-length cDNA ofzoa was 746 bp and contained a 510 bp open reading frame (ORF) encoding a lectin precursor of 169 amino acids with a signal peptide. ZOA was a mannose-binding lectin with three typical mannose-binding sites (QDNY). Semi-quantitative RT-PCR analysis revealed thatzoa expressed in all the tested tissues ofZ. officinale including leaf, root and rhizome, suggesting it to be a constitutively expressing form. ZOA protein was successfully expressed inEscherichia coli with the molecular weight expected. To our knowledge, this is the first mannose-binding lectin cDNA cloned from the family Zingiberaceae. Our results demonstrate that monocot mannose-binding lectins also occur within the family Zingiberaceae

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Abbreviations

AAA:

Allium ascalonicum agglutinin

ACPA:

Ananas comosus (pineapple) agglutinin

AKA:

Amorphophallus konjac agglutinin

APA:

Allium porrum agglutinin

ASA:

Allium sativum agglutinin

AUA:

Allium ursinum agglutinin

CAA:

Crinum asiaticum agglutinin

CHA:

Cymbidium hybrid agglutinin

EHA:

Epipactis helleborine agglutinin

GEAFP:

Gastrodia elata antifungal protein

GNA:

Galanthus nivalis agglutinin

LOA:

Listera ovata agglutinin

NHA:

Narcissus hybrid agglutinin

ORF:

open reading frame

PCR:

polymerase chain reaction

RACE:

rapid amplification of cDNA ends

RT-PCR:

reverse transcriptase-polymerase chain rection

TDA:

Typhonium divaricatum agglutinin

TMA:

Taxus media agglutinin

UTR:

untranslated region

ZGA:

Zephyrathes grandiflora agglutinin

ZOA:

Zingiber officinale agglutinin

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Correspondence to Kexuan Tang.

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Chen, Z., Kai, G., Liu, X. et al. cDNA cloning and characterization of a mannose-binding lectin fromZingiber officinaleRoscoe (ginger) rhizomes. J. Biosci. 30, 213–220 (2005). https://doi.org/10.1007/BF02703701

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Keywords

  • Mannose-binding lectin
  • RACE
  • Zingiber officinale
  • ZOA