Abstract
The hormonal modulation of thiamin carrier protein in the plasma and uterine luminal secretion during the normal reproductive phases of the animal (estrous cycle and pregnancy) as well as during experimental estrogenisation was investigated in the rat using a specific and sensitive homologous radioimmunoassay procedure developed for this purpose. Following a single injection of estrogen to immature male rats, thiamin carrier protein rapidly accumulated in plasma attaining peak concentration at 48 h and declining thereafter. A 1.5-fold amplification of the inductive response was observed on secondary stimulation with the hormone. The magnitude of the response exhibited a clear dependency on the dose of the steroid hormone, whereas the time at which peak levels of thiamin carrier protein production was remained unaltered in the concentration range of the steroid tested. The inductive effect of estrogen was severely curtailed by the antiestrogens,viz., En- and Zu-clomiphene citrates, while progesterone was incapable of either modulating the estrogen-induced response or eliciting an induction by itself. Cycloheximide drastically blocked the response to estrogen. Evidence for the ability of uterus to serve as yet another independent site of thiamin carrier protein synthesis was obtained byin vitro incorporation of radioactive amino acids into immunoprecipitable thiamin carrier protein in the tissue explants of estrogenised female rats. The levels of thiamin carrier protein in uterine luminal fluid measured during estrous cycle, pregnancy and experimental estrogenisation exhibited remarkable similarity to the plasma thiamin carrier protein profiles.
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Abbreviations
- E2 :
-
Estradiol 17β
- RCP:
-
riboflavin carrier protein
- TCP:
-
thiamine carrier protein
- RIA:
-
radioimmunoassay
- AH:
-
aminohexyl
- PBS:
-
sodium phosphate buffer, pH 7.2 containing 0.9 % NaCl
- BSA:
-
bovine serum albumin
- PMSF:
-
phenylmethylsulphonyl fluoride
- IgG:
-
immunoglobulin G
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Malathy, P.V., Adiga, P.R. Hormonal modulation of reproduction-specific thiamin carrier protein in the rat. J Biosci 7, 77–94 (1985). https://doi.org/10.1007/BF02703577
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DOI: https://doi.org/10.1007/BF02703577