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Differential reactivity of filarial antigens with human sera from bancroftian filariasis endemic zone

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Abstract

The reacting pattern of circulating filarial antigen fraction-2 fromWuchereria bancrofti and soluble antigen from adultBrugia malayi with bancroftian filarial sera were analysed by immunoblotting technique and enzyme linked immunosorbent assay. Microfilaraemic sera reacted specifically with proteins of molecular weight 200, 120, 97, 56, 54, 43, 26 and 17 kDa of circulating Filarial antigen fraction-2 and 44, 40, 38, 31, 22 and 18 kDa ofBrugia malayi adult soluble antigen. Clinical filarial sera identified protein molecules of 56, 54 and 42 kDa of circulating filarial antigen fraction-2 and 19, 16 and 14 kDa ofBrugia malayi adult soluble antigen. Some components of both the antigen preparation were also identified by endemic normal serai.e.proteins 120, 97, 62, 43 and 33 kDa of circulating filarial antigen fraction-2 and 170, 120, 43, 31 and 12 kDa ofBrugia malayi adult soluble antigen. One of the sodium dodecyl sulphate-polyacrylamide gel electropherosis fractions of circulating filarial antigen fraction-2 (CFA2-8) andBrugia malayi adult soluble antigen fraction-6 when used in enzyme linked immunosorbent assay could differentiate microfilaraemic sera from endemic normal and clinical filarial sera. The other antigen fractions (CFA2-2, 6 and 7 andBmA-2) showed a high geometric mean titre of filarial immunoglobulin G antibodies in endemic normal sera when compared to microfilaraemia and clinical filarial sera. These proteins need to be further studied to assess their involvement in protecting from filarial infection in an endemic area.

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Abbreviations

CFA2 :

Circulating filarial antigen fraction-2

BmA SDS-S Ag:

Brugia malayi adult SDS soluble antigen

ELISA:

enzyme linked immunosorbent assay

NCP:

nitrocellulose paper

SMP:

skimmed milk powder

CAM:

cellulose acetate membrane

GMT:

geometrical mean titre

References

  • Aggarwal A, Cuna W, Haque A, Dissous C and Capron A 1985 Resistance againstBrugia malayi microfilariae induced by a monoclonal antibody which promotes killing by macrophages and recognizes surface antigen(s);Immunology 54 655–663

    PubMed  CAS  Google Scholar 

  • Avrameas S 1969 Coupling of enzymes to proteins with glutaraldehyde—use of conjugates for the detection of antigens and antibodies;Immunochemistry 6 43–52

    Article  PubMed  CAS  Google Scholar 

  • Cheirmaraj K, Reddy MVR and Harinath B C 1990 Diagnostic use of polyclonal antibodies raised in mouse ascitic fluid in bancroftian filariasis;J. Immunoassay 11 429–444

    Article  PubMed  CAS  Google Scholar 

  • Dissanayake S, Forsyth K P, Ismail M M, Mitchell G F 1984 Detection of circulating antigen in bancroftian filariasis by using a monoclonal antibody;Am. J. Trop. Med. Hyg. 33 1130–1140

    PubMed  CAS  Google Scholar 

  • Forsyth K P, Spark R, Kazura J, Brown C V, Peters P, Heywood P, Dissanayake S and Mitchell G F 1985 A monoclonal antibody based immuno-radiometric assay for detection of circulating antigen in bancroftian filariasis;J. Immunol. 134 1172–1177

    PubMed  CAS  Google Scholar 

  • Freedman D O, Nutman T B and Ottesen E A 1989 Protective immunity in bancroftian filariasis. Selective recognition of a 43 kD larval stage antigen by infection-free individuals in an endemic area;J. Clin. Invest. 83 14–22

    Article  PubMed  CAS  Google Scholar 

  • Guellaen G, Goodhardtt M and Hanoune J 1984 Preparative SDS gel electrophoresis; inReceptor purification procedures (eds) J Craigventer and L Harrison (New York: Alan R Liss) Vol. 2, pp 109–124

    Google Scholar 

  • Hammerberg B, Nogami S, Nakagaki K, Hayashi Y and Tanaka H 1989 Protective immunity againstBrugia malayi infective larvae in mice. II. Induction by a T cell-dependent antigen isolated by monoclonal antibody affinity chromatography and SDS-PAGE;J. Immunol. 143 4201–4207

    PubMed  CAS  Google Scholar 

  • Kaushal N A, Hussain R and Ottesen E A 1984 Excretory-secretory and somatic antigens in the diagnosis of human filariasis;Clin. Exp. Immunol. 56 567–576

    PubMed  CAS  Google Scholar 

  • Kazura J W, Cicirello H and Forsyth K 1986 Differential recognition of a protective filarial antigen by antibodies from humans with bancroftian filariasis;J. Clin. Invest. 77 1985–1992

    PubMed  CAS  Google Scholar 

  • Kharat I, Cheirmaraj K, Prasad G B K S and Harinath B C 1989 Antigenic analysis of excretory-secretory products ofWuchereria bancrofti andBrugia malayi infective larval forms by SDS-PAGE;Indian J. Exp. Biol. 27 681–684

    PubMed  CAS  Google Scholar 

  • Lal R D, Paranjape R S, Briles D E, Nutman T B and Ottesen E A 1987 Circulating parasite antigen(s) in lymphatic filariasis: Use of monoclonal antibodies to phosphocholine for immunodiagnosis;J. Immunol. 138 3454–3460

    PubMed  CAS  Google Scholar 

  • Lammie P J, Eberhard M L and Lowrie R C Jr 1990 Differential humoral and cellular immuno-reactivity to saline and detergent extracted filarial antigens;Trans. R. Soc. Trop. Med. Hyg. 84 407–410

    Article  PubMed  CAS  Google Scholar 

  • Lowry O H, Rosebrough N J, Farr A L and Randall R J 1951 Protein measurement with the Folin-phenol reagent;J. Biol. Chem. 193 265–275

    PubMed  CAS  Google Scholar 

  • Lunde M N, Paranjape R, Lawley T J and Ottesen E A 1988 Filarial antigen in circulating immune complexes from patients withWuchereria bancrofti filariasis;Am. J. Trop. Med. Hyg. 38 366–371

    PubMed  CAS  Google Scholar 

  • Maizels R M, Sutanto I, Gomez-Priego A, Lillywhite J and Denham D A 1985 Specificity of surface molecules of adultBrugia parasites: cross-reactivity with antibody fromWuchereria, Onchocerca and other human filarial infections;Trop. Med. Parasitol. 36 233–237

    PubMed  CAS  Google Scholar 

  • Maizels R M, Burke J, Sutanto I, Purnomo, Partono F 1986 Secreted and surface antigens from larval stages ofWuchereria bancrofti, the major human lymphatic filarial parasite;Mol. Biochem. Parasitol. 19 27–34

    Article  PubMed  CAS  Google Scholar 

  • Nanduri J and Kazura J W 1989 Paramyosin-enhanced clearance ofBrugia malayi microfilaremia in mice;J. Immunol. 143 3359–3363

    PubMed  CAS  Google Scholar 

  • Ottesen E A, Weiler P F, Lunde M N and Hussain R 1982 Endemic filariasis on a Pacific Island. I Immunologie aspects: immunoglobulin, complement and specific antifilarial IgG, IgM and IgE antibodies;Am. J. Trop. Med. Hyg. 31 953–961

    PubMed  CAS  Google Scholar 

  • Parab P B, Rajasekariah G R, Carvalho P A and Subrahmanyam D 1990 Differential recognition ofBrugia malayi antigens by bancroftian filariasis sera;Indian /Med. Res. 91 138–143

    CAS  Google Scholar 

  • Paranjape R S, Hussain R, Nutman T B, Hamilton R and Ottesen E A 1986 Identification of circulating parasite antigen in patients with bancroftian filariasis;Clin. Exp. Immunol. 63 508–516

    PubMed  CAS  Google Scholar 

  • Parkhe K A, Reddy MVR, Cheirmaraj K, Ramaprasad P and Harinath B C 1990 Analysis, characterization and diagnostic use of circulating filarial antigen in bancroftian filariasis;J. Biosci. 15 37–46

    Google Scholar 

  • Reddy MVR, Prasad G B K S and Harinath B C 1986 Isolation and evaluation of antigens from microfilaraemia plasma and immune-complexes for diagnosis of bancroftian filariasis;Indian J. Pathol. Microbiol. 29 179–183

    PubMed  CAS  Google Scholar 

  • Sim K L, Kwa B H and Mak J W 1982 Immune responses in humanBrugia malayi infections: Serum dependent cell-mediated destruction of infective larvaein vitro;Trans. R. Soc. Trop. Med. Hyg. 76 362–370

    Article  PubMed  CAS  Google Scholar 

  • Subrahmanyam D, Mehta K, Nelson D S, Rao Y V B G and Rao C K 1978 Immune reactions in human filariasis;J. Clin. Microbiol. 8 228–232

    PubMed  CAS  Google Scholar 

  • Towbin M, Stachlin T and Gordon J 1979 Electrophoretic transfer of proteins from Polyacrylamide gels to nitro-cellulose sheets—Procedure and some applications;Proc. Natl. Acad. Sci. USA 76 4350–4354

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, 442 102, Sevagram, India

    K. Cheirmaraj, M. V. R. Reddy & B. C. Harinath

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  1. K. Cheirmaraj
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  2. M. V. R. Reddy
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  3. B. C. Harinath
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Cheirmaraj, K., Reddy, M.V.R. & Harinath, B.C. Differential reactivity of filarial antigens with human sera from bancroftian filariasis endemic zone. J Biosci 16, 199–208 (1991). https://doi.org/10.1007/BF02703285

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  • DOI: https://doi.org/10.1007/BF02703285

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