Abstract
In situ digestion of metaphase and polytene chromosomes and of interphase nuclei in different cell types ofDrosophila nasuta with restriction enzymes revealed that enzymes like AluI, EcoRI, HaeIII, Sau3a and SinI did not affect Giemsa-stainability of heterochromatin while that of euchromatin was significantly reduced; TaqI and SalI digested both heterochromatin and euchromatin in mitotic chromosomes. Digestion of genomic DNA with AluI, EcoRI, HaeIII, Sau3a and KpnI left a 23 kb DNA band undigested in agarose gels while withTaqI, no such undigested band was seen. TheAluI resistant 23 kb DNA hybridized insitu specifically with the heterochromatic chromocentre. It appears that the digestibility of heterochromatin region in genome ofDrosophila nasuta with the tested restriction enzymes is dependent on the availability of their recognition sites.
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References
Babu A 1988 Heterogeneity of heterochromatin of human chromosomes as demonstrated by restriction endonuclease treatment; inHeterochromatin-molecular and structural aspects (ed.) R S Verma (Cambridge: Cambridge University Press) pp 250–275
Bianchi M S, Bianchi N O, Pantelias G E and Wolff S 1985 The mechanism and patterns of banding induced by restriction endonucleases in human chromosomes;Chromosoma 91 131–136
Burkholder G D 1989 Morphological and biochemical effects of endonucleases on isolated mammalian chromosomesin vitro;Chromosoma 98 347–355
Hammond M P and Laird C D 1985 Chromosome structure and DNA replication in nurse and follicle cellsof Drosophila melanogaster;Chromosoma 91 279–296
Hatsumi M, Morishige V and Wakahama K I 1988 Metaphase chromosomes of four species of theDrosophila nasuta subgroup;Jpn. J. Genet. 63 435–444
Holmquist G P 1989 Evolution of chromosome bands: molecular ecology of noncoding DNA;J. Mol. Evol. 28 469–486
Kumar M and Lakhotia S C 1977 Localization of non-replicating heterochromatin in polytene cells ofDrosophila nasuta by fluorescence microscopy;Chromosoma 59 301–305
Lakhotia S C 1984 Replication inDrosophila chromosomes. XII. Reconfirmation of under replication of heterochromatin in polytene nuclei by cytofluorometry;Chromosoma 89 63–67
Lakhotia S C and Kumar M 1978 Heterochromatin in mitotic chromosomes ofDrosophila nasuta;Cytobios 21 79–89
Lakhotia S C and Roy J K 1981 Effect of Hoechst 33258 on condensation patterns of hetero- and euchromatin in mitotic and interphase nucleiofDrosophila nasuta;Exp. Cell. Res. 132 423–431
Lakhotia S C and Roy J K 1983 Effects of distamycin A and netropsin on condensation of mitotic chromosomes in early embryos and larval brain cells ofDrosophila;Indian J. Exp. Biol. 21 357–362
Lakhotia S C and Roy S 1979 Replication inDrosophila chromosomes. I. Replication of intranucleolar DNA in polytene cells ofD.nasuta;J. Cell Sci. 36 185–197
Lakhotia S C, Roy J K and Kumar M 1979 A study of heterochromatin inDrosophila nasuta by the 5-bromodeoxyuridine-Giemsa staining technique;Chromosoma 72 249–255
Lima-de-Faria A, Isaksson M and Olsson E 1980 Action of restriction endonuclease on the DNA and chromosomesofMuntiacus muntjak;Hereditas 92 267–273
Lohe A and Roberts P 1988 Evolution of satellite DNA sequences inDrosophila; inHeterochromatin — molecular and structural aspects (ed.) R S Verma (Cambridge: Cambridge University Press) pp 148–156
Lopez-Fernandez C, Gosalvez J, Suja J A and Mezzanotte R 1989 Restriction endonuclease digestion of meiotic and mitotic chromosomes inPyrgomorpha conica (Orthoptera, Pyrogomorphidae);Genome 30 621–626
Maniatis T, Fritsch E F and Sambrook J 1983Molecular cloning. A laboratory manual (Cold Spring Harbor: Cold Spring Harbor Laboratory)
Mezzanotte R 1986 The selective digestion of polytene and mitotic chromosomes ofDrosophila melanogaster by theAluI andHaeIII restriction endonucleases;Chromosoma 93 249–255
Mezzanotte R, Manconi P E and Ferruci L 1986 On the possibility of localizingin situ Mus musculus andDrosophila virilis satellite DNAs byAluI andEcoRI restrict endonucleases;Genetica 70 107–111
Miller D A, Choi J C and Miller O J 1983 Chromosome localization of highly repetitive human DNAs and amplified ribosomal DNA with restriction enzymes;Science 219 395–397
Miller D A and Miller O J 1990 Restriction enzyme banding of metaphase chromosomes; inTrends in chromosome research (ed..) T Sharma (Berlin: Springer-Verlag and New Delhi: Narosa) pp 238–250
Pardue M L 1986 In situ hybridization to DNA of chromosomes and nuclei; inDrosophila—a practical approach (ed.) D B Robberts (Oxford, Washington: IRL Press) pp 111–137
Raman R and Lakhotia S C 1990 Comparative aspects of chromosome replication inDrosophila and mammals; inTrends in chromosome research (ed.) T Sharma (Berlin: Springer-Verlag and New Delhi: Narosa) pp 69–89
Ranganath H A, Schmidt E R and Hagele K 1982 Satellite DNA ofDrosophila nasuta andD. n. albomicana: localization in polytene and metaphase chromosomes:Chromosoma 85 361–368
Spradling A and Orr-Weaver T 1988 Regulation of replication duringDrosophila development;Annu. Rev. Genet. 21 373–403
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Tiwari, P.K., Lakhotia, S.C. Restriction enzyme digestion of heterochromatin inDrosophila nasuta . J Biosci 16, 187–197 (1991). https://doi.org/10.1007/BF02703284
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DOI: https://doi.org/10.1007/BF02703284